...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >BROAD RESISTANCE TO PLANT VIRUSES IN TRANSGENIC PLANTS CONFERRED BY ANTISENSE INHIBITION OF A HOST GENE ESSENTIAL IN S-ADENOSYLMETHIONINE-DEPENDENT TRANSMETHYLATION REACTIONS
【24h】

BROAD RESISTANCE TO PLANT VIRUSES IN TRANSGENIC PLANTS CONFERRED BY ANTISENSE INHIBITION OF A HOST GENE ESSENTIAL IN S-ADENOSYLMETHIONINE-DEPENDENT TRANSMETHYLATION REACTIONS

机译：反义抑制S-腺苷甲硫氨酸依赖的反甲基化反应赋予宿主基因必需的转基因植物对植物病毒的抗性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

S-Adenosylhomocysteine hydrolase (SAHH) is a key enzyme in transmethylation reactions that use S-adenosylmethionine as the methyl donor. Because of the importance of SAHH in a number of S-adenosylmethionine-dependent transmethylation reactions, particularly the 5' capping of mRNA during viral replication, SAHH has been considered as a target of potential antiviral agents against animal viruses. To test the possibility of engineering a broad type of resistance to plant viruses, we expressed the antisense RNA for tobacco SAHH in transgenic tobacco plants. As expected, transgenic plants constitutively expressing an antisense SAHH gene showed resistance to infection by various plant viruses. Among those plants, about half exhibited some level of morphological change (typically stunting). Analysis of the physiological change in those plants showed that they contained excess levels of cytokinin. Because cytokinin has been found to induce acquired resistance, there is also a strong possibility that the observed resistance was induced by cytokinin. [References: 30]
机译：S-腺苷同型半胱氨酸水解酶（SAHH）是使用S-腺苷甲硫氨酸作为甲基供体的甲基化反应中的关键酶。由于SAHH在许多S-腺苷甲硫氨酸依赖的甲基化反应中的重要性，尤其是病毒复制过程中mRNA的5'帽化，因此SAHH被认为是针对动物病毒的潜在抗病毒药物的靶标。为了测试工程化对植物病毒的广泛耐药性的可能性，我们在转基因烟草植物中表达了烟草SAHH的反义RNA。如预期的那样，组成型表达反义SAHH基因的转基因植物表现出对各种植物病毒感染的抵抗力。在这些植物中，约有一半表现出一定程度的形态变化（通常为发育迟缓）。对那些植物的生理变化的分析表明，它们含有过量的细胞分裂素。因为已经发现细胞分裂素诱导获得性抗性，所以观察到的抗性很可能是由细胞分裂素诱导的。 [参考：30]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号