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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >IDENTIFICATION OF THE BACILLUS SUBTILIS PUR OPERON REPRESSOR
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IDENTIFICATION OF THE BACILLUS SUBTILIS PUR OPERON REPRESSOR

机译：枯草芽孢杆菌Pur OPERON阻遏物的鉴定

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Transcription of the Bacillus subtilis pur operon is repressed in response to a signal of excess adenine, We have purified the repressor protein and have identified, cloned, and overexpressed the purR regulatory gene that controls transcription initiation of the operon. B. subtilis purR encodes a 62-kDa homodimer that binds to the pur operon control region. The PurR binding site which overlaps the promoter encompasses approximate to 110 bp. The protein-DNA interaction is inhibited by 5-phosphoribosyl 1-pyrophosphate. A mutation that deletes the repressor binding site or one that disrupts purR abolishes binding activity in vitro and repression of transcription in vivo in response to the excess adenine signal. These results lead to a model in which an excess-adenine signal is transmitted to PurR via the 5-phosphoribosyl 1-pyrophosphate pool. In addition, purR is autoregulated. There is no structural or mechanistic similarity between the B. subtilis and Escherichia coli purine repressors. [References: 29]
机译：响应过量腺嘌呤的信号，抑制枯草芽孢杆菌pur操纵子的转录。我们已经纯化了阻遏蛋白，并鉴定，克隆并过表达了控制操纵子转录起始的purR调节基因。枯草芽孢杆菌purR编码与嘌呤操纵子控制区结合的62 kDa同型二聚体。与启动子重叠的PurR结合位点涵盖约110bp。蛋白质-DNA相互作用被5-磷酸核糖基1-焦磷酸抑制。删除阻遏物结合位点的突变或破坏purR的突变，将在体外消除结合活性，并响应过量的腺嘌呤信号而在体内抑制转录。这些结果导致了一个模型，其中过量的腺嘌呤信号通过5-磷酸核糖基1-焦磷酸池传递到PurR。另外，purR是自动调节的。枯草芽孢杆菌和大肠杆菌嘌呤阻遏物之间没有结构或机制的相似性。 [参考：29]

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