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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >AN ARABIDOPSIS SYNTAXIN HOMOLOGUE ISOLATED BY FUNCTIONAL COMPLEMENTATION OF A YEAST PEP12 MUTANT
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AN ARABIDOPSIS SYNTAXIN HOMOLOGUE ISOLATED BY FUNCTIONAL COMPLEMENTATION OF A YEAST PEP12 MUTANT

机译：酵母PEP12突变体功能互补分离的拟南芥突触素同源物

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摘要

The syntaxin family of integral membrane proteins are thought to function as receptors for transport vesicles, with different isoforms of this family localized to various membranes throughout the cell, The yeast Pep12 protein is a syntaxin homologue which may function in the trafficking of vesicles from the trans-Golgi network to the vacuole, We have isolated an Arabidopsis thaliana cDNA by functional complementation of a yeast pep12 mutant. The Arabidopsis cDNA (aPEP12) potentially encodes a 31-kDa protein which is homologous to yeast Pep12 and to other members of the syntaxin family, indicating that this protein may function in the docking or fusion of transport vesicles with the vacuolar membrane in plant cells, Northern blot analysis indicates that the mRNA is expressed in all tissues examined, although at a very low level in leaves, The mRNA is found in all cell types in roots and leaves, as shown by in situ hybridization experiments, The existence of plant homologues of proteins of the syntaxin family indicates that the basic vesicle docking and fusion machinery may be conserved in plants as it is in yeast and mammals. [References: 41]
机译：完整膜蛋白的syntaxin家族被认为是转运小泡的受体，该家族的不同同种型位于整个细胞的各种膜上。酵母Pep12蛋白是syntaxin的同源物，可能在反式转运小泡的过程中起作用-高尔基体到液泡的网络，我们通过酵母pep12突变体的功能互补分离了拟南芥cDNA。拟南芥cDNA（aPEP12）可能编码一种31-kDa蛋白，该蛋白与酵母Pep12和Syntaxin家族的其他成员同源，表明该蛋白可能在植物细胞中液泡膜的转运小泡对接或融合中起作用， Northern印迹分析表明，尽管在叶片中的mRNA含量很低，但在所有检测的组织中都表达了mRNA。如原位杂交实验所示，在根部和叶片的所有细胞类型中均发现了mRNA。 Syntaxin家族的蛋白质表明，基本的囊泡对接和融合机制在酵母和哺乳动物中可能在植物中得以保留。 [参考：41]

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