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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >TARGETED DISRUPTION OF VINCULIN GENES IN F9 AND EMBRYONIC STEM CELLS CHANGES CELL MORPHOLOGY, ADHESION, AND LOCOMOTION
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TARGETED DISRUPTION OF VINCULIN GENES IN F9 AND EMBRYONIC STEM CELLS CHANGES CELL MORPHOLOGY, ADHESION, AND LOCOMOTION

机译：F9和胚胎干细胞中长春新碱基因的靶标破坏会改变细胞的形态，粘附和运动

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摘要

Vinculin, a major constituent of focal adhesions and zonula adherens junctions, is thought to be involved in linking the microfilaments to areas of cell-substrate and cell-cell contacts, To test the role of vinculin in cell adhesion and motility, we used homologous recombination to generate F9 embryonal carcinoma and embryonic stem cell clones homozygous for a disrupted vinculin gene, When compared to wild-type cells, vinculin-mutant cells displayed a rounder morphology and a reduced ability to adhere and spread on plastic or fibronectin, Decreased adhesion of the mutant cells was associated with a reduction in lamellipodial extensions, as observed by time-lapse video microscopy. The locomotive activities of control F9 and the vinculin-null cells were compared in two assays. Loss of vinculin resulted in a 2.4-fold increase in cell motility. These results demonstrate an important role for vinculin in determining cell shape, adhesion, surface protrusive activity, and cell locomotion. [References: 36]
机译：Vinculin是粘着斑和小带粘附连接的主要成分，被认为与微丝连接到细胞底物和细胞间接触区域有关。为了测试纽菌素在细胞粘附和运动中的作用，我们使用了同源重组产生F9胚胎癌和胚胎干细胞克隆的纯合蛋白基因被纯合。与野生型细胞相比，连合蛋白突变细胞表现出更圆的形态，在塑料或纤连蛋白上的黏附和扩散能力降低，黏附力降低延时视频显微镜观察到，突变细胞与lamellipodial延伸减少有关。在两种试验中比较了对照F9和链蛋白-空细胞的机车活性。长链蛋白的损失导致细胞运动性增加2.4倍。这些结果证明了纽蛋白在确定细胞形状，粘附，表面突出活性和细胞运动中的重要作用。 [参考：36]

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