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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >PLANT MEMBERS OF A FAMILY OF SULFATE TRANSPORTERS REVEAL FUNCTIONAL SUBTYPES
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PLANT MEMBERS OF A FAMILY OF SULFATE TRANSPORTERS REVEAL FUNCTIONAL SUBTYPES

机译：硫酸盐转运蛋白家族功能亚型的植物成员

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摘要

Three plant sulfate transporter cDNAs have been isolated by complementation of a yeast mutant with a cDNA library derived from the tropical forage legume Stylosanthes hamata. Two of these cDNAs, shst1 and shst2, encode high-affinity H+/sulfate cotransporters that mediate the uptake of sulfate by plant roots from low concentrations of sulfate in the soil solution, The third, shst3, represents a different subtype encoding a lower affinity H+/sulfate cotransporter, which may be involved in the internal transport of sulfate between cellular or subcellular compartments within the plant. The steady-state level of mRNA corresponding to both subtypes is subject to regulation by signals that ultimately respond to the external sulfate supply. These cDNAs represent the identification of plant members of a family of related sulfate transporter proteins whose sequences exhibit significant amino acid conservation in filamentous fungi, yeast, plants, and mammals. [References: 28]
机译：通过将酵母突变体与衍生自热带牧草豆科植物Stylosanthes hamata的cDNA文库互补，已分离出三种植物硫酸盐转运蛋白cDNA。这些cDNA中的两个，shst1和shst2，编码高亲和力的H + /硫酸盐共转运体，介导植物根系从土壤溶液中低浓度的硫酸盐吸收硫酸盐，第三个，shst3，代表编码较低亲和力H +的不同亚型。 /硫酸盐共转运蛋白，其可能参与植物中细胞或亚细胞区室之间硫酸盐的内部运输。对应于两种亚型的mRNA的稳态水平受最终对外部硫酸盐供应反应的信号的调节。这些cDNA代表了相关硫酸盐转运蛋白家族的植物成员的鉴定，其序列在丝状真菌，酵母，植物和哺乳动物中显示出显着的氨基酸保守性。 [参考：28]

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