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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A plant virus-encoded protein facilitates long-distance movement of heterologous viral RNA
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A plant virus-encoded protein facilitates long-distance movement of heterologous viral RNA

机译:植物病毒编码的蛋白质可促进异源病毒RNA的长距离移动

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摘要

Transport of plant viruses from cell to cell typically involves one or more viral proteins that supply specific cell-to-cell movement functions. Long-distance trans- port of viruses through the vascular system is a less well understood process with requirements different from those of cell-to-cell movement. Usually viral coat protein (CP) is required for long-distance movement, but groundnut rosette umbravirus (GRV) does not code for a CP. However, this virus moves efficiently from cell to cell and long distance. We demonstrate here that the protein encoded by ORF3 of GRV can functionally replace the CP of tobacco mosaic virus (TMV) for long-distance movement. In spite of low levels of virus RNA accumulation in infected cells, chimeric TMV with a replace- ment of the CP gene by GRV ORF3 was able to move rapidly through the phloem. Moreover, this chimeric virus comple- mented long-distance movement of another CP-deficient TMV derivative expressing the gene encoding the green fluorescent protein. Thus, the GRV OM3-encoded protein represents a class of trans-acting long-distance movement factors that can facilitate trafficking of an unrelated viral RNA.
机译:植物病毒从细胞到细胞的运输通常涉及一种或多种提供特定细胞间移动功能的病毒蛋白。病毒通过血管系统的长距离运输是一个鲜为人知的过程,其要求不同于细胞到细胞的运动。通常,长距离运动需要病毒外壳蛋白(CP),但花生花丛伞病毒(GRV)并不编码CP。但是,这种病毒可以有效地从一个细胞移动到另一个细胞,并且距离很长。我们在这里证明GRV的ORF3编码的蛋白质可以在功能上替代烟草花叶病毒(TMV)的CP,以进行长距离运动。尽管病毒RNA在感染细胞中的积累水平较低,但嵌合的TMV被GRV ORF3取代了CP基因,它能够在韧皮部中快速移动。此外,这种嵌合病毒还补充了另一种CP缺陷型TMV衍生物的长距离运动,后者表达了编码绿色荧光蛋白的基因。因此,GRV OM3编码的蛋白代表了一类可促进不相关病毒RNA转运的反式长距离运动因子。

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