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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis
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Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis

机译：GDP-甘露糖在植物抗坏血酸（维生素C）生物合成中作用的遗传证据

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摘要

Vitamin C (t-ascorbic acid3; AsA) acts as a potent antioxidant and cellular reductant in plants and animals. AsA has long been known to have many critical physiological roles in plants, yet its biosynthesis is only currently being defined. A pathway for AsA biosynthesis that features GDP-mannose and L-galactose has recently been proposed for plants. We have isolated a collection of AsA. deFicient mutants of Arabidopsis thaliana that are valuable tools for testing of an AsA biosynthetic Pathway. The hest- characterized of these mutants (vtcl) contains approx. = of wild- type AsA and is defective in AsA biosynthesis. By using a combination of biochemical, molecular, and genetic tech- niques, we have demonstrated that the ryCI locus encodes a GDP.mannose pyrophosphorylase (mannose-1-p guanyl- transferase). This enzyme provides GDP-mannose, which is used for cell wall carbohydrate biosynthesis and protein glycosylation as well as for AsA biosynthesis. In addition to genetically defining the first locus involved in AsA biosynthe- sis, this work highlights the power of using traditional mu- tagenesis techniques coupled with the Arabidopsis Gcnome Initiative to rapidly clone physiologically important genes.
机译：维生素C（抗坏血酸3； AsA）在植物和动物中可作为有效的抗氧化剂和细胞还原剂。众所周知，AsA在植物中具有许多关键的生理作用，但目前仅对其生物合成进行了定义。最近已经提出了一种以GDP-甘露糖和L-半乳糖为特征的AsA生物合成途径。我们已经隔离了AsA的集合。拟南芥的deFicient突变体，是测试AsA生物合成途径的宝贵工具。这些突变体（vtcl）的特征-含有约。 ＝野生型AsA的α，并且在AsA生物合成中有缺陷。通过结合使用生化，分子和遗传技术，我们证明了ryCI基因座可编码GDP。甘露糖焦磷酸化酶（甘露糖-1-p鸟苷转移酶）。这种酶提供了GDP-甘露糖，可用于细胞壁碳水化合物的生物合成和蛋白质糖基化以及AsA的生物合成。除了从遗传上定义涉及AsA生物合成的第一个基因座外，这项工作还强调了使用传统诱变技术与拟南芥Gcnome Initiative结合以快速克隆重要生理基因的能力。

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