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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Spc29p is a component of the Spcllop subcomplex and is essential for spindle pole body duplication
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Spc29p is a component of the Spcllop subcomplex and is essential for spindle pole body duplication

机译：Spc29p是Spcllop子复合体的组成部分，对于主轴极体复制至关重要

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摘要

In yeast, microtubules are organized by the spindle pole body (SPB). The SPB is a disk-like multilayered structure that is embedded in the nuclear envelope via its central plaque, whereas the outer and inner plaques are exposed to the cytoplasm and nucleoplasm, respectively. How the SPB assembles is poorly understood. We show that the inner/central plaque is composed of a stable SPB subcomplex, containing the #gamma#-tubulin complex-binding protein Spcll0p, calmodulin, Spc41p, and Spc29p. Spc29p acts as a linker between the central plaque component Spc41p and the inner plaque protein Spcll0p. Evidence is provided that the cal- modulin-binding site of Spcll0p influences the binding of Spc29p to Spcl10p. Spc41p also was identified as a component of a cytoplasmic SPB subcomplex containing Spc94p/Nudlp, Cnm67p, and Spc41p. Spc29p and Spc41p may be part of a critical interface of nucleoplasmic and cytoplasmic assembled SPB subcomplexes that form during SPB duplication. In agreement with this, overexpressed Spc29p was found to be a nuclear protein, whereas Spc41p is cytoplasmic. In addition, an essential function of SPC29 during SPB assembly is indicated by the SPB duplication defect of conditional lethal spc29(ts) cells and by the genetic interaction of SPC29 with CDC31 and KAR1, two genes that are involved in SPB dupli- cation.
机译：在酵母中，微管由纺锤极体（SPB）组成。 SPB是一种盘状多层结构，通过其中心斑块嵌入核膜中，而外部和内部斑块分别暴露于细胞质和核质。 SPB的组装方式知之甚少。我们显示内部/中央斑块由稳定的SPB亚复合体组成，包含＃gamma＃-微管蛋白复合体结合蛋白Spcll0p，钙调蛋白，Spc41p和Spc29p。 Spc29p充当中央噬菌斑成分Spc41p和内部噬菌斑蛋白Spcll0p之间的连接子。有证据表明Spc110p的钙调蛋白结合位点会影响Spc29p与Scl10p的结合。 Spc41p还被确定为包含Spc94p / Nudlp，Cnm67p和Spc41p的胞质SPB亚复合体的组成部分。 Spc29p和Spc41p可能是在SPB复制过程中形成的核质和细胞质组装SPB亚复合体关键界面的一部分。与此一致，发现过表达的Spc29p是一种核蛋白，而Spc41p是胞质的。此外，条件性致死性spc29（ts）细胞的SPB复制缺陷以及SPC29与CDB31和KAR1的遗传相互作用表明了SPC29在SPB组装过程中的基本功能，这两个基因参与了SPB复制。

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