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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Regulation of RpoS proteolysis in Escherichia coit: The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS
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Regulation of RpoS proteolysis in Escherichia coit: The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS

机译：大肠杆菌中RpoS蛋白水解的调节：响应调节剂RssB是与RpoS中营业额元素相互作用的识别因子

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The degradation of the RpoS (#sigma#~s) subunit of RNA polymerase in Escherichia coli is a prime example of regulated proteolysis in prokaryotes. RpoS turnover depends on ClpXP protease, the response regulator RssB, and a hitherto uncharacterized "turnover element" within RpoS itself. Here we localize the turnover element to a small element (around the crucial amino acid lysine-173) directly down- stream of the promoter-recognizing region 2.4 in RpoS. Its sequence as well as its location identify the turnover element as a unique proteolysis-promoting motif. This element is shown to be a site of interaction with RssB. Thus, RssB is functionally unique among response regulators as a direct recognition factor in ClpXP-dependent RpoS proteolysis. Binding of RssB to RpoS is stimulated by phosphorylation of the RssB receiver domain, suggesting that environmental stress affects RpoS proteolysis by modulating RssB affinity for RpoS. Initial evidence indicates that lysine-173 in RpoS, besides being essential of RpoS proteolysis, may play a role in promoter recognition. Thus the same region in RpoS is crucial for proteolysis as well as for activity as a transcription factor.
机译：大肠杆菌中RNA聚合酶RpoS（s）的亚单位的降解是原核生物中受调节的蛋白水解的主要例子。 RpoS营业额取决于ClpXP蛋白酶，响应调节剂RssB和RpoS本身迄今未表征的“营业额要素”。在这里，我们将周转元件定位在RpoS中启动子识别区2.4的直接下游的一个小元件（关键氨基酸赖氨酸173周围）。它的序列及其位置将周转元件确定为独特的蛋白水解促进基序。该元素显示为与RssB交互的站点。因此，在依赖于ClpXP的RpoS蛋白水解中，RssB在反应调节剂中是功能独特的，是直接识别因子。 RssB受体域的磷酸化刺激RssB与RpoS的结合，表明环境胁迫通过调节RssB对RpoS的亲和力影响RpoS的蛋白水解。初步证据表明，RpoS中的赖氨酸173除了是RpoS蛋白水解的必要条件外，还可能在启动子识别中起作用。因此，RpoS中的同一区域对于蛋白水解以及作为转录因子的活性至关重要。

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