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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Receptors for oxidized low-density lipoprotein on elicited mouse peritoneal macrophages can recognize both the modified lipid moieties and the modified protein moieties:Implications with respect to macrophage recognition of apoptotic cells
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Receptors for oxidized low-density lipoprotein on elicited mouse peritoneal macrophages can recognize both the modified lipid moieties and the modified protein moieties:Implications with respect to macrophage recognition of apoptotic cells

机译：诱发的小鼠腹膜巨噬细胞上氧化型低密度脂蛋白的受体可以识别修饰的脂质部分和修饰的蛋白质部分：对凋亡细胞的巨噬细胞识别的意义

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It has been shown previously that the binding of oxidized low-density lipoprotein (OxLDL) to resident mouse peritoneal macrophages can be inhibited (up to 70) by the apoprotein B (apoB) isolated from OxLDL, suggesting that macrophage recognition of OxLDL is primarily depen- dent on its modified protein moiety. However, recent experi- ments have demonstrated that the lipids isolated from OxLDL and reconstituted into a microemulsion can also strongly inhibit uptake of OxLDL (up to 80). The present studies show that lipid microemulsions prepared from OxLDL bind to thioglycollate-elicited macrophages at 4 deg C in a saturablc fashion and inhibit the binding of intact OxLDL and also of the apoB from OxLDL. Reciprocally, the binding of the OxLDL-lipid microemulsions was strongly inhibited by intact OxLDL. A conjugate of synthetic 1-palmitoyl 2 (5-oxovaleroyl) phosphatidylcholine (an oxidation product of 1-palmitoyl 2-arachidonoyl phosphatidylcholine) with serum albumin, shown previously to inhibit macrophage binding of intact OxLDL, also inhibited the binding of both the apoprotein and the lipid microemulsions prepared from OxLDL. Finally, a monoclonal antibody against oxidized phospholipids, one that inhibits binding of intact OxLDL to macrophages, also inhib- ited the binding of both the resolubilized apoB and the lipid microemulsions prepared from OxLDL. These studies sup- port the conclusions that: (i) at least some of the macrophage reccptors for oxidized LDL can recognize both the lipid and the protein moieties, and (ii) oxidized phospholipids, in the lipid phase of the
机译：先前已证明，从OxLDL分离的脱辅基蛋白B（apoB）可以抑制（低至70）氧化的低密度脂蛋白（OxLDL）与驻留的小鼠腹膜巨噬细胞的结合，这表明主要依赖于OxlDL的巨噬细胞识别-凹痕其修饰的蛋白质部分。但是，最近的实验表明，从OxLDL中分离出来的脂质并重构为微乳状液也可以强烈抑制OxLDL的摄取（最多80种）。本研究表明，由OxLDL制备的脂质微乳以饱和的方式与硫代乙醇酸酯诱导的巨噬细胞在4摄氏度下结合，并抑制完整的OxLDL以及与OxLDL的apoB的结合。相反，完整的OxLDL强烈抑制OxLDL-脂质微乳剂的结合。合成的1-棕榈酰基2（5-氧戊酰基）磷脂酰胆碱（1-棕榈酰基2-花生四烯酰基磷脂酰胆碱的氧化产物）与血清白蛋白的结合物，先前显示抑制完整的OxLDL的巨噬细胞结合，也抑制脱辅基蛋白和载脂蛋白的结合由OxLDL制备的脂质微乳。最后，一种抗氧化磷脂的单克隆抗体（抑制完整的OxLDL与巨噬细胞结合）也抑制了再溶解的apoB和由OxLDL制备的脂质微乳剂的结合。这些研究支持以下结论：（i）至少一些用于氧化LDL的巨噬细胞受体可以识别脂质和蛋白质部分，以及（ii）脂质相中的氧化磷脂。

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