Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors

Dwight D. Koeberl; Ian E. Alexander; Christine L. Halbert

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Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors

机译：静脉注射腺相关病毒载体后小鼠肝脏中人凝血因子的持久表达

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We previously found that gene transduction by adeno-associated virus (AAV) vectors in cell culture can be stimulated over 100-fold by treatment of the target cells with agents that affect DNA metabolism, such as irradiation or topoisomerase inhibitors. Here we show that previous γ-irra- diation increased the transduction rate in mouse liver by up to 900-fold, and the topoisomerase inhibitor etoposide in- creased transduction by about 20-fold. Similar rates of hepatic transduction were obtained by direct injection of the liver or by systemic delivery via tail vein injection.

机译：我们先前发现，通过用影响DNA代谢的试剂（例如辐射或拓扑异构酶抑制剂）处理靶细胞，可以在细胞培养物中将腺相关病毒（AAV）载体的基因转导刺激100倍以上。在这里，我们表明先前的γ辐照使小鼠肝脏中的转导率提高了900倍，而拓扑异构酶抑制剂依托泊苷将转导率提高了约20倍。通过直接注射肝脏或通过尾静脉注射全身递送获得相似的肝转导率。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第4期|p.1426-1431|共6页
作者
Dwight D. Koeberl; Ian E. Alexander; Christine L. Halbert;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Optimized adeno-associated virus (AAV)-protein phosphatase-5 helper viruses for efficient liver transduction by single-stranded AAV vectors: therapeutic expression of factor IX at reduced vector doses. [J] . Jayandharan GR, Zhong L, Sack BK, Human gene therapy . 2010,第3期

机译：优化的腺相关病毒（AAV）-蛋白磷酸酶5辅助病毒，可通过单链AAV载体有效地进行肝脏转导：减少载体剂量时因子IX的治疗性表达。
2. Efficient delivery of human clotting factor IX after injection of lentiviral vectors in utero [J] . Xiao-guang CHEN, Huan-zhang ZHU, Ju-li GONG, Acta Pharmacologica Sinica . 2004,第6期

机译：在子宫内注射慢病毒载体后有效递送人凝血因子
3. Comparison of Gene Delivery to the Kidney by Adenovirus, Adeno-Associated Virus, and Lentiviral Vectors After Intravenous and Direct Kidney Injections [J] . Rubin Jeffrey D., Nguyen Tien V., Allen Kari L., Human gene therapy . 2019,第12期

机译：静脉内和直接肾注射后腺病毒，腺瘤相关病毒和慢病毒载体对肾的基因递送与肾病
4. Adenovirus-mediated regulataile Expression of human Factor IX In vitro and in wiwo [C] . M.A. Srour, H. Fechner, X Wang, Hemophilie-Symposion . 2003

机译：腺病毒介导人体因子IX在体外和Wiwo中的临床表达
5. Prime-boost vaccine strategies using adenovirus and adeno-associated virus vectors to induce CD8+ T cell responses against human immunodeficiency virus type I [D] . Lin, Shih-Wen (Wenny) 2008

机译：使用腺病毒和腺伴随病毒载体的初免-加强疫苗策略诱导针对I型人免疫缺陷病毒的CD8 + T细胞应答
6. Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors [O] . Dwight D. Koeberl, Ian E. Alexander, Christine L. Halbert, 1997

机译：静脉注射腺相关病毒载体后小鼠肝脏中人凝血因子的持久表达
7. Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors [O] . Koeberl, Dwight D., Alexander, Ian E., Halbert, Christine L., 1997

机译：静脉注射腺相关病毒载体后小鼠肝脏中人凝血因子的持久表达
8. Persistent and High-Level Expression of Human Liver Prolidase in Vivo in Mice Using Adenovirus. [R] . Aleti, V., Reddy, G. B., Parikh, K., 2013

机译：使用腺病毒在小鼠体内持续和高水平表达人肝脏prolidase。

Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors

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