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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >GALACTOSE-DEPENDENT REVERSIBLE INTERACTION OF GAL3P WITH GAL80P IN THE INDUCTION PATHWAY OF GAL4P-ACTIVATED GENES OF SACCHAROMYCES CEREVISIAE
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GALACTOSE-DEPENDENT REVERSIBLE INTERACTION OF GAL3P WITH GAL80P IN THE INDUCTION PATHWAY OF GAL4P-ACTIVATED GENES OF SACCHAROMYCES CEREVISIAE

机译：GAL3P与GAL80P的半乳糖依赖性可逆相互作用在酿酒酵母中GAL4P激活的基因的诱导途径中。

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摘要

When galactose is added to logarithmically growing culture of the yeast Saccharomyces cerevisiae, a set of genes encoding galactose-metabolizing enzymes (GAL genes) starts to be transcribed within a few minutes, This rapid induction involves a serial interplay of Gal3p, Gal80p, and Gal4p, Recent experiments have indicated that a direct interaction between Gal3p and Gal80p plays a pivotal role in an early step of GAL induction, Here we demonstrate that complex of Gal3p and GalSOp, otherwise unstable, is stabilized in the presence of 0.1 mM galactose and 0.5 mM ATP, The requirement for galactose and ATP for stable complex formation is also observed by using highly purified Gal3p and Gal80p from yeast. We further show that thus formed Gal3p/Gal80p complex can easily be dissociated when it is washed with buffer lacking galactose, Finally, we show that mutant proteins encoded by GAL80(S) or GAL80DE21, which confer galactose-uninducible phenotype, fail to interact with Gal3p. These results strongly suggest that Gal3p functions as the sensor and transducer of galactose signal in the induction pathway of Gal4p-activated genes. [References: 38]
机译：当将半乳糖添加到酵母酿酒酵母的对数生长培养物中时，编码半乳糖代谢酶（GAL基因）的一组基因开始在几分钟内被转录，这种快速诱导涉及Gal3p，Gal80p和Gal4p的一系列相互作用最近的实验表明，Gal3p和Gal80p之间的直接相互作用在GAL诱导的早期过程中起着关键作用。在这里，我们证明了Gal3p和GalSOp的复合物（否则不稳定）在存在0.1 mM半乳糖和0.5 mM的情况下稳定了。 ATP，通过使用来自酵母的高度纯化的Gal3p和Gal80p，也可以观察到对半乳糖和ATP稳定复合物形成的需求。我们进一步表明，这样形成的Gal3p / Gal80p复合物在用缺少半乳糖的缓冲液洗涤时很容易解离。最后，我们证明了由GAL80（S）或GAL80DE21编码的赋予半乳糖不可诱导表型的突变蛋白不能与之相互作用。 Gal3p。这些结果强烈表明，Gal3p在Gal4p激活基因的诱导途径中充当半乳糖信号的传感器和传感器。 [参考：38]

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