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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >MICROCOLINEARITY IN SH2-HOMOLOGOUS REGIONS OF THE MAIZE, RICE, AND SORGHUM GENOMES
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MICROCOLINEARITY IN SH2-HOMOLOGOUS REGIONS OF THE MAIZE, RICE, AND SORGHUM GENOMES

机译：玉米，大米和高粱基因组SH2-同种异体区域的微线性

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摘要

Large regions of genomic colinearity have been demonstrated among grass species by recombinational mapping, but the degree of chromosomal conservation at the sub-centimorgan level has not been extensively investigated, We cloned the rice and sorghum genes homologous to the sh2 locus of maize on bacterial artificial chromosomes (BACs), and observed that a homologue of the maize al gene was also present on each of these BACs, In sorghum, we found a direct duplication of al homologues separated by about 10 kb, In maize, sh2 and al are approximately 140 kb apart and transcribed in the same direction, with sh2 upstream of al, In rice and sorghum, this arrangement is fully conserved, However, the sh2 and al homologues are separated by about 19 kb in both rice and sorghum, We found low-copy-number and repetitive DNAs between the sh2 and al homologues of sorghum and rice, The sh2 and al homologues cross-hybridized, but the repetitive DNA and most low-copy-number sequences between these genes did not, These results indicate that maize, sorghum, and rice have conserved gene order and composition in the sh2-a1 region, but have acquired extensive qualitative and quantitative differences in the sequences between these genes. [References: 24]
机译：通过重组作图已在草种中证明了大范围的基因组共线性，但尚未广泛研究亚下生殖器官水平的染色体保守程度。我们通过细菌人工克隆克隆了与玉米sh2基因座同源的水稻和高粱基因染色体（BAC），并观察到每个玉米BAC上也存在玉米Al基因的同源物，在高粱中，我们发现Al同源物的直接重复大约相距10 kb，在玉米中，sh2和al约为140在水稻和高粱中，sh2和al的同系物被完全隔开，但在水稻和高粱中，sh2和al的同源物被大约19 kb隔开，我们发现低拷贝高粱和水稻的sh2和al同源物之间的数量和重复DNA，sh2和al的同源物交叉杂交，但是这些基因之间的重复DNA和大多数低拷贝数序列这些结果表明，玉米，高粱和水稻在sh2-a1区域保留了基因顺序和组成，但在这些基因之间的序列中获得了广泛的定性和定量差异。 [参考：24]

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