Maturation of Physarum mitochondrial RNA involves the highly specific insertion of nonencoded nucleo- tides at multiple locations. To investigate the mechanism(s) by which this occurs, we previously developed an isolated mito- chondrial system in which run-on transcripts are accurately and efficiently edited by nucleotide insertion. Here we show that under limiting concentrations of exogenous nucleotides the mitochondrial RNA polymerases stall, generating a pop- ulation of nascent RNAs that can be extended upon addition of limiting nucleotide.
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