A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: A novel defect in plasmalogen biosynthesis

Narasimhan Nagan; Amiya K. Hajra; Arun K. Das

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A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: A novel defect in plasmalogen biosynthesis

机译：烷基二羟基丙酮磷酸合酶有缺陷的成纤维细胞系：缩醛缩醛生物合成中的新缺陷

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摘要

Using fluorescence-activated cytotoxicity se- lection, followed by colony autoradiographic screening of the surviving population, we have isolated a unique plasmalogen- deficient Chinese hamster ovary (CHO) cell line. The mutant, NZel-1, showed a dramatic (90/100) reduction in the rate of biosynthesis and levels of plasmalogens, as determined using short- and long-term labeling with ~32Pi. Enzymatic assays and lipid supplementation studies showed that NZel-1 was defec- tive in a single step in the biosynthetic pathway for plasmalo- gens.

机译：使用荧光激活的细胞毒性选择，然后对存活的人群进行菌落放射自显影检查，我们分离出了一种独特的缺乏血浆蛋白的中国仓鼠卵巢（CHO）细胞系。突变体NZel-1在〜32Pi的短期和长期标记中显示，其生物合成速率和缩醛磷脂水平显着降低（90/100）。酶促测定和脂质补充研究表明，NZel-1在血浆生成素生物合成途径的一步中是缺陷。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第9期|p.4475-4480|共6页
作者
Narasimhan Nagan; Amiya K. Hajra; Arun K. Das;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Isolation of a Chinese hamster fibroblast variant defective in dihydroxyacetonephosphate acyltransferase activity and plasmalogen biosynthesis: use of a novel two-step selection protocol. [J] . Nagan N, Hajra AK, Larkins LK, The Biochemical Journal . 1998,第Pta1期

机译：中国仓鼠成纤维细胞变体在二羟基丙酮磷酸酯酰基转移酶活性和缩醛缩醛生物合成中的分离：使用新型的两步选择方案。
2. Isolation of a Chinese hamster fibroblast variant defective in dihydroxyacetonephosphate acyltransferase activity and plasmalogen biosynthesis: use of a novel two-step selection protocol [J] . Nagan N., Larkins LK., Lazarow P., The Biochemical Journal . 1998,第Pta1期

机译：二羟基丙酮磷酸酰基转移酶活性和缩醛缩醛生物合成缺陷的中国仓鼠成纤维细胞变体的分离：使用新型的两步选择方案
3. Isolation and characterization of mutant animal cell line defective in alkyl-dihydroxyacetonephosphate synthase: localization and transport of plasmalogens to post-Golgi compartments. [J] . Honsho M, Yagita Y, Kinoshita N Biochimica et biophysica acta. Molecular cell research . 2008,第10期

机译：烷基-二羟基丙酮磷酸合酶有缺陷的突变动物细胞系的分离和表征：缩醛磷脂的定位和运输至高尔基体后区室。
4. EFFECTS OF MANNOSE-6-PHOSPHATE ON HUMAN FIBROBLAST CELLS [C] . David A. Black, Alex Whittington, Tamika Taylor, 47th annual rocky mountain bioengineering symposium 47th international ISA biomedical sciences instrumentation symposium 2010 . 2010

机译：6-磷酸甘露聚糖对人成纤维细胞的影响
5. Studies of plasmalogen biosynthesis and regulation in animal cells: Insights using somatic cell mutants. [D] . Zheng, Hongying. 2005

机译：动物细胞缩醛磷脂生物合成与调控的研究：利用体细胞突变体的见解。
6. A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: A novel defect in plasmalogen biosynthesis [O] . Narasimhan Nagan, Amiya K. Hajra, Arun K. Das, 1997

机译：烷基二羟基丙酮磷酸合酶有缺陷的成纤维细胞系：缩醛缩醛生物合成中的新型缺陷
7. Isolation of a Chinese hamster fibroblast variant defective in dihydroxyacetonephosphate acyltransferase activity and plasmalogen biosynthesis: use of a novel two-step selection protocol. [O] . Nagan, N, Hajra, A K, Larkins, L K, 1998

机译：中国仓鼠成纤维细胞变异体的分离在二羟基丙酮磷酸酰基转移酶活性和缩醛缩醛生物合成方面存在缺陷：使用新型的两步选择方案。

A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: A novel defect in plasmalogen biosynthesis

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