Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin

Carmen Quinto; Andre H. M. Wijfjes; Guido V. Bloemberg

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Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin

机译：细菌结节蛋白NodZ是几丁质寡糖岩藻糖基转移酶，也可以识别动物来源的相关底物

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The nodZ gene, which is present in various soil bacteria such as Bradyrhizobium japonicum, Azorhizobium cauli- nodans, and Rhizobium loti, is involved in the addition of a fucosyl residue to the reducing N-acetylglucosamine residue of lipochitin oligosaccharide (LCO) signal molecules. Using an Escherichia coli strain that produces large quantities of the NodZ protein of B. japonicum, we have purified the NodZ protein to homogeneity. The purified NodZ protein appears to be active in an in vitro transfucosylation assay in which GDP-β-fucose and LCOs or chitin oligosaccharides are used as substrates.

机译：存在于各种土壤细菌中的nodZ基因，例如日本短枝根瘤菌，菜豆根瘤菌和Lothi根瘤菌，都涉及在岩藻糖基寡糖（LCO）信号分子的还原N-乙酰氨基葡萄糖残基上添加岩藻糖基残基。使用产生大量日本根瘤菌NodZ蛋白的大肠杆菌菌株，我们已将NodZ蛋白纯化至同质。纯化的NodZ蛋白似乎在体外转岩藻糖基化测定中具有活性，其中GDP-β-岩藻糖和LCOs或几丁质寡糖被用作底物。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第9期|p.4336-4341|共6页
作者
Carmen Quinto; Andre H. M. Wijfjes; Guido V. Bloemberg;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor [J] . Brzezinski K, Stepkowski T, Panjikar S, Acta Biochimica Polonica . 2007,第3期

机译：NodZ岩藻糖基转移酶的高分辨率结构参与结瘤因子的生物合成
2. High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor [J] . Acta biochimica Polonica . 2007,第3期

机译：NodZ岩藻糖基转移酶的高分辨率结构参与结瘤因子的生物合成
3. Rhizobium sp. strain NGR234 NodZ protein is a fucosyltransferase. [J] . D Quesada-Vincens, R Fellay, T Nasim, Journal of bacteriology . 1997,第16期

机译：根瘤菌NGR234菌株NodZ蛋白是岩藻糖基转移酶。
4. Characterization of Bacterial Protein Glycosylation Intermediates by LC-MS/MS on Porous Graphitic Carbon: Discovery of C_(45) and C_(60) Polyisoprenyl-oligosaccharide Lipid Carriers [C] . Jacek Stupak, Christopher W. Reid, Christine M. Szymanski, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：通过LC-MS / MS对多孔石墨碳的细菌蛋白糖基化中间体的表征：C_（45）和C_（60）聚异戊二烯基 - 寡糖脂质载体的发现
5. Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin [O] . Carmen Quinto, André H. M. Wijfjes, Guido V. Bloemberg, 1997

机译：细菌结节蛋白NodZ是几丁质寡糖岩藻糖基转移酶也可以识别动物来源的相关底物
6. High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor. [O] . Krzysztof Brzezinski, Tomasz Stepkowski, Santosh Panjikar, 2007

机译：Nodz岩藻糖基转移酶的高分辨率结构涉及结缔组织的生物合成因子。
7. Removal of Eutrophic Nutrients from Wastewater and their Bioconversion to Bacterial Single Cell Protein for Animal Feed Supplements. Phase III [R] . DeCicco, B. T. 1980

机译：从废水中去除富营养化物质及其生物转化为动物饲料添加剂的细菌单细胞蛋白质。第三阶段

Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin

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