Characterization of human telomerase complex

Shyam Ramakrishnan; Harsh W. Sharma; A. Darise Farris

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Characterization of human telomerase complex

机译：人端粒酶复合物的表征

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Telomerase, a ribonucleoprotein complex, adds hexameric repeats called "telomeres" to the growing ends of chromosomal DNA. Characterization of mammalian telomerase has been elusive because of its low level of expres- sion. We describe a bioinformatics approach to enrich and characterize the human telomerase complex. Using local sequence homology search methods, we detected similarity of the Tetrahymena p80 subunit of telomerase with the autoan- tigen Ro60. Antibodies to Ro60 immunoprecipitated the te- lomerase activity. Ro60 and p80 proteins were cross- recognizable by antibodies to either protein.

机译：端粒酶是一种核糖核蛋白复合物，它在染色体DNA的生长末端添加称为“端粒”的六聚体重复序列。哺乳动物端粒酶的表达水平较低，因此难以捉摸。我们描述了丰富和表征人类端粒酶复合物的生物信息学方法。使用局部序列同源性搜索方法，我们检测到端粒酶四膜虫p80亚基与自身抗原Ro60的相似性。 Ro60抗体可免疫沉淀端粒酶活性。 Ro60和p80蛋白可以通过针对这两种蛋白的抗体进行交叉识别。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第19期|p.10075-10079|共5页
作者
Shyam Ramakrishnan; Harsh W. Sharma; A. Darise Farris;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells. [J] . Laetitia Trapp-Fragnet, Delphine T Marie-Egyptienne, Johans Fakhoury, Cellular & molecular biology letters. . 2012,第4期

机译：人端粒酶催化亚基和病毒端粒酶RNA可在无细胞系统中而非人细胞中重建功能性端粒酶复合物。
2. The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells [J] . Chantal Autexier, Delphine T. Marie-Egyptienne, Denis Rasschaert, Cellular & molecular biology letters. . 2012,第4期

机译：人端粒酶催化亚基和病毒端粒酶RNA可在无细胞系统中重组功能性端粒酶复合物，但在人细胞中则不能
3. Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes [J] . Robert Alexander Wu, Yavuz S Dagdas, S Tunc Yilmaz, eLife journal . 2015,第september期

机译：人端粒酶全酶和最小RNP复合物中端粒酶逆转录酶的单分子成像
4. Effects of different doses of X-ray on telomerase in human osteosarcoma cells (HOS cells) [C] . Wang Lijun, Qian Honggang, Li Ming, 2011 International Conference on Human Health and Biomedical Engineering . 2011

机译：不同剂量的X射线对人骨肉瘤细胞（HOS细胞）端粒酶的影响
5. Conserved and Divergent Mouse and Human Telomerase and Telomere Regulation: Implications for the Development and Validation of Telomerase and Telomere-Specific Anticancer Strategies. [D] . Fakhoury, Johans. 2010

机译：保守和发散的小鼠和人类端粒酶和端粒调节：对端粒酶和端粒特异性抗癌策略的发展和验证的影响。
6. The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system but not in human cells [O] . Laetitia Trapp-Fragnet, Delphine T. Marie-Egyptienne, Johans Fakhoury, 2012

机译：人端粒酶催化亚基和病毒端粒酶RNA在无细胞系统中重构功能性端粒酶复合物但在人细胞中则不能
7. The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells [O] . Laetitia Trapp-Fragnet, Delphine Marie-Egyptienne, Johans Fakhoury, 2012

机译：人端粒酶催化亚基和病毒端粒酶RNA在无细胞系统中重建功能性端粒酶复合物，但不在人体细胞中

Characterization of human telomerase complex

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