Subunit rotation in Escherichia coli F_oF_1-ATP synthase during oxidative phosphorylation

Yuantai Zhou; Thomas M. Duncan; Richard L. Cross

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Subunit rotation in Escherichia coli F_oF_1-ATP synthase during oxidative phosphorylation

【24h】

Subunit rotation in Escherichia coli F_oF_1-ATP synthase during oxidative phosphorylation

机译：氧化磷酸化过程中大肠杆菌F_oF_1-ATP合酶中的亚基旋转

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We report evidence for proton-driven sub- unit rotation in membrane-bound F_oF_1-ATP synthase during oxidative phosphorylation. A βD380C/γC87 crosslinked hy- brid F_1 having epitope-tagged βD380C subunits (βflag) exclu- sively in the two noncrosslinked positions was bound to Fo in F1-depleted membranes. After reduction of the β-γ crosslink, a brief exposure to conditions for ATP synthesis followed by reoxidation resulted in a significant amount of β_flag appearing in the β-γ crosslinked product. Such a reorientation of γC87 relative to the three β subunits can only occur through subunit rotation. Rotation was inhibited when proton transport through F_o was blocked or when ADP and Pi were omitted. These results establish F_oF_1 as the second example in nature where proton transport is coupled to subunit rotation.

机译：我们报告了膜结合的F_oF_1-ATP合酶在氧化磷酸化过程中质子驱动的亚单位旋转的证据。在两个未交联位置上都具有表位标记的βD380C亚基（β标记）的βD380C/γC87交联杂交F_1与贫F1膜中的Fo结合。 β-γ交联键还原后，短暂暴露于ATP合成条件后再氧化，导致在β-γ交联产物中出现大量β_flag。 γC87相对于三个β亚基的这种重新定向只能通过亚基旋转来实现。当质子通过F 0的传输被阻止或省略ADP和Pi时，旋转受到抑制。这些结果将F_oF_1作为质子传输与亚基旋转耦合的第二个实例。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第20期|p.10583-10587|共5页
作者
Yuantai Zhou; Thomas M. Duncan; Richard L. Cross;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词

相似文献

外文文献
中文文献
专利

1. ATP synthase from Escherichia coli: Mechanism of rotational catalysis, and inhibition with the epsilon subunit and phytopolyphenols [J] . Biochimica et biophysica acta. Bioenergetics . 2016,第2期

机译：大肠杆菌中的ATP合酶：旋转催化的机理，以及ε亚基和植物多酚的抑制作用
2. Conformational changes in the alpha-subunit coupled to binding of the beta2-subunit of tryptophan synthase from Escherichia coli: crystal structure of the tryptophan synthase alpha-subunit alone. [J] . Nishio K, Morimoto Y, Ishizuka M, Biochemistry . 2005,第4期

机译：与大肠杆菌色氨酸合酶β2-亚基结合结合的α-亚基构象变化：仅色氨酸合酶α-亚基的晶体结构。
3. The F1F0-ATPase of Escherichia coli. Substitution of proline by leucine at position 64 in the c-subunit causes loss of oxidative phosphorylation [J] . A E Senior, A L Fimmel, D A Jans, The biochemical journal . 1983,第2期

机译：大肠杆菌的F1F0-ATPase。 c亚基第64位的亮氨酸取代脯氨酸会导致氧化磷酸化丧失
4. Subunit rotation in a single F_oF_1-ATP synthase in a living bacterium monitored by FRET [C] . K. Seyfert, T. Oosaka, H. Yaginuma, Single molecule spectroscopy and imaging IV . 2011

机译：FRET监测活细菌中单个F_oF_1-ATP合酶中的亚基旋转
5. Proton Transport at the Interface of Subunit a and the Rotor Ring of Escherichia coli ATP Synthase [D] . Steed, P. Ryan 2010

机译：质子在亚单位a和转子ATP合酶的转子环的界面上的运输。
6. Subunit rotation in Escherichia coli FoF1–ATP synthase during oxidative phosphorylation [O] . Yuantai Zhou, Thomas M. Duncan, Richard L. Cross 1997

机译：氧化磷酸化过程中大肠杆菌FoF1-ATP合酶中的亚基旋转
7. Energy-driven subunit rotation at the interface between subunit a and the c oligomer in the FO sector of Escherichia coli ATP synthase [O] . Hutcheon, Marcus L., Duncan, Thomas M., Ngai, Helen, 2001

机译：大肠杆菌ATP合酶FO区段中亚基a和c低聚物之间界面处的能量驱动亚基旋转
8. Comparison of an ATPase from the Archaebacterium Halobacterium Saccharovorum with the F1 Moiety from the Escherichia Coli ATP Synthase [R] . Stan-Lotter, H., Hochstein, L. I. 1989

机译：来自saccharovorum的古细菌的aTp酶与大肠杆菌aTp合成酶的F1部分的比较

Subunit rotation in Escherichia coli F_oF_1-ATP synthase during oxidative phosphorylation

摘要

著录项

相似文献

相关主题

期刊订阅