Probing the assembly of transcription initiation complexes through changes in σ~N protease sensitivity

Paul Casaz; Martin Buck

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Probing the assembly of transcription initiation complexes through changes in σ~N protease sensitivity

机译：通过改变σ〜N蛋白酶敏感性来探测转录起始复合物的组装

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The alternative bacterial σ~N RNA polymer- ase holoenzyme binds promoters as a transcriptionally inac- tive complex that is activated by enhancer-binding proteins. Little is known about how sigma factors respond to their ligands or how the responses lead to transcription. To examine the liganded state of σ~N, the assembly of end-labeled Klebsiella pneumoniae σ~N into holoenzyme, closed promoter complexes, and initiated transcription complexes was analyzed by enzy- matic protein footprinting. V8 protease-sensitive sites in free σ~N were identified in the acidic region II and bordering or within the minimal DNA binding domain.

机译：备选的细菌σ〜N RNA聚合酶全酶将启动子结合为转录不活跃复合物，并被增强子结合蛋白激活。关于σ因子如何响应其配体或响应如何导致转录，人们所知甚少。为了检查σ〜N的配体状态，通过酶促蛋白质印迹法分析了末端标记的肺炎克雷伯菌σ〜N组装成全酶，封闭的启动子复合物和起始的转录复合物。在酸性区II中，在最小DNA结合域的边界或内部，发现了游离σ〜N中的V8蛋白酶敏感位点。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第22期|p.12145-12150|共6页
作者
Paul Casaz; Martin Buck;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates. [J] . J A Knezetic, G A Jacob, D S Luse Molecular and Cellular Biology . 1988,第8期

机译：在核小体组装之前组装RNA聚合酶II预启动复合物，可以在核小体模板上有效地启动转录。
2. GNE-371, a Potent and Selective Chemical Probe for the Second Bromodomains of Human Transcription-Initiation-Factor TFIID Subunit 1 and Transcription-Initiation-Factor TFIID Subunit 1-like [J] . Wang Shumei, Tsui Vickie, Crawford Terry D., Journal of Medicinal Chemistry . 2018,第20期

机译：GNE-371，一种有效和选择性化学探针，用于第二溴染色剂的人转录引发因子TFIID亚基1和转录起始因子TFIID亚基1-lik
3. GNE-371, a Potent and Selective Chemical Probe for the Second Bromodomains of Human Transcription-Initiation-Factor TFIID Subunit 1 and Transcription-Initiation-Factor TFIID Subunit 1-like [J] . Wang Shumei, Tsui Vickie, Crawford Terry D., Journal of Medicinal Chemistry . 2018,第20期

机译：GNE-371，一种有效和选择性化学探针，用于第二溴染色剂的人转录引发因子TFIID亚基1和转录起始因子TFIID亚基1-lik
4. Probing the oligomeric state of the chloroplast proteome in Arabidopsis thaliana by mass spectrometry: megadalton-sized complexes and the Clp protease machinery [C] . Paul Dominic Olinares, Lalit Ponnala, Klaas J. van Wijk American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：用质谱探测拟南芥丙烯酸氯化体蛋白质组的低聚状态：Megadalton大小复合物和CLP蛋白酶机械
5. The Function of an Alternate TFB from Pyrococus furiosus and the Orientation of the TFB B-reader within Archaeal Transcription Initiation Complexes [D] . Micorescu, Michael 2010

机译：激烈热球菌替代TFB的功能和古细菌转录起始复合物中TFB B阅读器的方向。
6. Probing the assembly of transcription initiation complexes through changes in σN protease sensitivity [O] . Paul Casaz, Martin Buck 1997

机译：通过改变σN蛋白酶的敏感性探测转录起始复合物的组装
7. Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates. [O] . Knezetic, J A, Jacob, G A, Luse, D S 1988

机译：在核小体组装之前组装RNA聚合酶II预启动复合物，可以在核小体模板上有效地启动转录。

Probing the assembly of transcription initiation complexes through changes in σ~N protease sensitivity

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