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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Killing K Channels with TEA~+
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Killing K Channels with TEA~+

机译：用TEA杀死K频道〜+

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摘要

Tetraethylammonium (TEA~+) is widely used for reversible blockade of K channels in many preparations. We noticed that intracellular perfusion of voltage-clamped squid giant axons with a solution containing K~+ and TEA~+ irreversibly decreased the potassium current when there was no K~+ outside. Five minutes of perfusion with 20 mM TEA~+, followed by removal of TEA~+, reduced potassium current to <5/100 of its initial value. The irreversible disappearance of K channels with TEA+ could be prevented by addition of ≥ 10 mM K~+ to the extracellular solution. The rate of disappear- ance of K channels followed first-order kinetics and was slowed by reducing the concentration of TEA~+.
机译：四乙基铵（TEA〜+）在许多制剂中广泛用于可逆性阻断K通道。我们注意到，当外部没有K〜+时，电压钳制的鱿鱼巨轴突的含K〜+和TEA〜+溶液的细胞内灌注不可逆地降低了钾电流。用20 mM TEA〜+灌注5分钟，然后去除TEA〜+，将钾电流降至其初始值的<5/100。在细胞外液中加入≥10 mM K〜+可以防止TEA +导致K通道不可逆消失。钾通道的消失速率遵循一级动力学，并通过降低TEA〜+的浓度而减慢。

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