Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination

Vladimir Larionov; Natalya Kouprina; Joan Graves

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Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination

机译：通过转化相关重组特异性克隆人类DNA作为酵母人工染色体

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DNA molecules undergoing transformation into yeast are highly recombinogenic, even when diverged. We reasoned that transformation-associated recombination (TAR) could be employed to clone large DNAs containing repeat sequences, thereby eliminating the need for in vitro enzymatic reactions such as restriction and ligation and reducing the amount of DNA handling. Gently isolated human DNA was transformed directly into yeast spheroplasts along with two genetically marked (M1 and M2) linearized vectors that contained a human Alu sequence at one end and a telomere sequence at the other end (Alu-CEN-M1-TEL and Alu-M2-TEL).

机译：经历转化为酵母的DNA分子即使在发生分歧时也具有很高的重组原性。我们认为可以采用转化相关重组（TAR）克隆包含重复序列的大DNA，从而消除了对体外酶促反应（如限制酶和连接酶）的需要，并减少了DNA处理量。轻轻分离的人类DNA与两个遗传标记的线性化载体（M1和M2）一起直接转化到酵母原生质球中，线性化载体的一端包含一个人Alu序列，另一端包含一个端粒序列（Alu-CEN-M1-TEL和Alu- M2-TEL）。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第1期|p.491-496|共6页
作者
Vladimir Larionov; Natalya Kouprina; Joan Graves;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning [J] . Vladimir Larionov, Natalya Kouprina, Joan Graves Proceedings of the National Academy of Sciences of the United States of America . 1996,第24期

机译：通过转化相关重组克隆从啮齿动物-人杂种细胞（作为环状酵母人工染色体）中高度选择性地分离人DNA
2. Cloning of human centromeres by transformation-associated recombination in yeast and generation of functional human artificial chromosomes [J] . N. Kouprina, T. Ebersole, M. Koriabine, Nucleic Acids Research . 2003,第3期

机译：通过酵母中的转化相关重组克隆人着丝粒并产生功能性人类人工染色体
3. A novel expression system for genomic DNA loci using a human artificial chromosome vector with transformation-associated recombination cloning. [J] . Ayabe F, Katoh M, Inoue T, Journal of human genetics . 2005,第11期

机译：一种新型的基因组DNA基因座表达系统，使用具有转化相关重组克隆的人类人工染色体载体。
4. elopment of flow cytogenetics for construction of chromosome and chromosome arm-specific DNA libraries in bread wheat. [C] . J. Safar, J. Janda, J. Bartos, International Wheat Genetics Symposium . 2003

机译：流动细胞遗传学措施施工染色体和染色体特异性DNA文库中的面包小麦。
5. Fully human antigen-specific polyclonal antibody responses induced in cloned human artificial chromosome transchromosomic cattle. [D] . Choi, Yoon Jong. 2005

机译：在克隆的人类人工染色体转染色体牛中诱导的完全人类抗原特异性多克隆抗体反应。
6. Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination. [O] . V Larionov, N Kouprina, J Graves, 1996

机译：通过转化相关重组将人类DNA特异性克隆为酵母人工染色体。
7. Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination. [O] . Larionov, V, Kouprina, N, Graves, J, 1996

机译：通过转化相关重组将人类DNA特异性克隆为酵母人工染色体。
8. Human chromosome 21: Linkage mapping and cloning in yeast artificial chromosomes. Progress report. [R] . Antonarakis, S. E. 1991

机译：人类染色体21：酵母人工染色体中的连锁图谱和克隆。进度报告。

Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination

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