Multiplex selection technique (MuST): An approach to clone transcription factor binding sites

Girish N. Nallur; Kulkarni Prakash; Sherman M. Weissman

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Multiplex selection technique (MuST): An approach to clone transcription factor binding sites

机译：多重选择技术（MuST）：一种克隆转录因子结合位点的方法

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We have used a multiplex selection approach to construct a library of DNA-protein interaction sites rec- ognized by many of the DNA-binding proteins present in a cell type. An estimated minimum of two-thirds of the binding sites present in a library prepared from activated Jurkat T cells represent authentic transcription factor binding sites. We used the library for isolation of "optimal" binding site probes that facilitated cloning of a factor and to identify binding activities induced within 2 hr of activation of Jurkat cells.

机译：我们已经使用了多重选择的方法来构建一个DNA-蛋白质相互作用位点的文库，该库被细胞类型中存在的许多DNA结合蛋白所识别。由活化的Jurkat T细胞制备的文库中估计存在的结合位点的至少三分之二代表真实的转录因子结合位点。我们使用该文库来分离“最佳”结合位点探针，以促进因子的克隆并鉴定在激活Jurkat细胞后2小时内诱导的结合活性。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第3期|p.1184-1189|共6页
作者
Girish N. Nallur; Kulkarni Prakash; Sherman M. Weissman;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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2. An approach to predict transcription factor DNA binding site specificity based upon gene and transcription factor functional categorization [J] . Ziliang Qian, Lingyi Lu, XiaoJun Liu, Bioinformatics . 2007,第18期

机译：基于基因和转录因子功能分类的预测转录因子DNA结合位点特异性的方法
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4. Simultaneous Identification for Synergistic Transcription Factors and their Transcription Factor Binding Sites [C] . Tsai Zing Tsung-Yeh, Huang Grace Tzu-Wei, Tsai Huai-Kuang 2011 International Conference on Complex, Intelligent and Software Intensive Systems . 2011

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5. Novel optical techniques for exploring the DNA binding affinity and specificity of eukaryotic transcription factors and transcription factor complexes. [D] . Bonham, Andrew James. 2010

机译：探索真核转录因子和转录因子复合物的DNA结合亲和力和特异性的新型光学技术。
6. Multiplex selection technique (MuST): an approach to clone transcription factor binding sites. [O] . G N Nallur, K Prakash, S M Weissman 1996

机译：多重选择技术（MuST）：一种克隆转录因子结合位点的方法。
7. Multiplex selection technique (MuST): an approach to clone transcription factor binding sites. [O] . Nallur, G N, Prakash, K, Weissman, S M 1996

机译：多重选择技术（MuST）：一种克隆转录因子结合位点的方法。
8. DNA Binding Drugs Targeting the Regulatory DNA Binding Site of the ETS Domain Family Transcription Factor Associated with Human Breast Cancer [R] . Wang, Y. 2001

机译：靶向与人乳腺癌相关的ETs结构域家族转录因子的调节DNa结合位点的DNa结合药物

Multiplex selection technique (MuST): An approach to clone transcription factor binding sites

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