Purification and physical properties of the male and female double sex proteins of Drosophila

Sayeon Cho; Pieter C. Wensink

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Purification and physical properties of the male and female double sex proteins of Drosophila

机译：果蝇雄性和雌性双重性蛋白的纯化和物理性质

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At higher concentrations, the proteins form tetramers. Through use of a novel, double crosslinking assay (protein-DNA plus protein-protein), we demonstrated that a DNA regulatory site binds to both monomers of the DSX dimer and to only two monomers of the tetramer. Further- more, binding another DNA molecule to what we presume is the second and identical site in the tetramer dramatically shifts the equilibrium from tetramers to dimers. These oli- gomerization and DNA binding properties are indistinguish- able between the male and female proteins.

机译：在较高浓度下，蛋白质形成四聚体。通过使用新型的双交联测定法（蛋白质-DNA加蛋白质-蛋白质），我们证明了DNA调控位点与DSX二聚体的两个单体以及四聚体的仅两个单体结合。此外，将另一个DNA分子与我们假定的结合是四聚体中的第二个相同位点，从而使平衡从四聚体变为二聚体。这些低聚和DNA结合特性在雄性和雌性蛋白之间是无法区分的。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第5期|p.2043-2047|共5页
作者
Sayeon Cho; Pieter C. Wensink;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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6. Purification and physical properties of the male and female double sex proteins of Drosophila. [O] . S Cho, P C Wensink 1996

机译：果蝇雄性和雌性双性蛋白的纯化和物理性质。
7. Purification and physical properties of the male and female double sex proteins of Drosophila. [O] . Cho, S, Wensink, P C 1996

机译：果蝇雄性和雌性双性蛋白的纯化和物理性质。
8. Biochemical and Physical Properties of Stored Male and Female Donor Blood [R] . DeVenuto, F., Ligon, D. F., Jukes, K. H., 1970

机译：存储的男性和女性供体血液的生化和物理特性

Purification and physical properties of the male and female double sex proteins of Drosophila

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