Sequence specificity of triplex DNA formation: Analysis by a combinatorial approach, restriction endonuclease protection selection and amplification

Paul Hardenbol; Michael W. Van Dyke

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Sequence specificity of triplex DNA formation: Analysis by a combinatorial approach, restriction endonuclease protection selection and amplification

机译：三链体DNA形成的序列特异性：通过组合方法进行分析，限制性核酸内切酶保护选择和扩增

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We have devised a combinatorial method, restriction endonuclease protection selection and amplifica- tion (REPSA), to identify consensus ligand binding sequences in DNA. In this technique, cleavage by a type IIS restriction endonuclease (an enzyme that cleaves DNA at a site distal from its recognition sequence) is prevented by a bound ligand while unbound DNA is cleaved. Since the selection step of REPSA is performed in solution under mild conditions, this approach is amenable to the investigation of ligand-DNA complexes that are either insufficiently stable or not readily separable by other methods.

机译：我们设计了一种组合方法，限制性核酸内切酶保护选择和扩增（REPSA），以鉴定DNA中的共有配体结合序列。在该技术中，当未结合的DNA被切割时，结合的配体阻止了IIS型限制性核酸内切酶（一种在其识别序列远端的位点切割DNA的酶）的切割。由于REPSA的选择步骤是在温和的条件下在溶液中进行的，因此该方法适用于研究配体-DNA复合物，这些复合物要么稳定性不足，要么不易通过其他方法分离。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第7期|p.2811-2816|共6页
作者
Paul Hardenbol; Michael W. Van Dyke;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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2. Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis [J] . Hideo Takahashi, Hiroyuki Ohshima, Nobutaka Hirano Nucleic acids research . 2006,第17期

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3. Sequence-specific protection of plasmid DNA from restriction endonuclease hydrolysis by pyrrole-imidazole-cyclopropapyrroloindole conjugates [J] . Kazuhisa Fujimoto, Hirokazu Iida, Masako Kawakami, Nucleic Acids Research . 2002,第17期

机译：吡咯-咪唑-环丙吡咯并吲哚偶联物对质粒DNA的限制性核酸内切酶水解的序列特异性保护
4. Examining Flanking Sequence Specificity and Topological Specificity in the Binding of Various Molecular Types to DNAs Using Restriction Endonuclease Activity Assays [C] . S. A. Winkle, E. Duran, J. Pulido, Symposium on frontiers in nucleic acids . 2011

机译：使用限制性内切核酸酶活性测定检查各种分子类型与DNA的结合中的侧翼序列特异性和拓扑特异性
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机译：限制性内切酶Eco RI：其内在金属中心以及与DNA识别位点的特定相互作用（蛋白-核酸，锌金属酶，相互作用的手性金属络合物，DNA结合蛋白）。
6. Sequence specificity of triplex DNA formation: Analysis by a combinatorial approach restriction endonuclease protection selection and amplification. [O] . P Hardenbol, M W Van Dyke 1996

机译：三链DNA形成的序列特异性：通过组合方法进行分析限制性核酸内切酶保护选择和扩增。
7. Sequence specificity of triplex DNA formation: Analysis by a combinatorial approach, restriction endonuclease protection selection and amplification. [O] . Hardenbol, P, Van Dyke, M W 1996

机译：三链DNA形成的序列特异性：通过组合方法进行分析，限制性核酸内切酶保护选择和扩增。
8. Specificity of Polymerase Chain Amplification Reactions for Human Immunodeficiency Virus Type 1 DNA Sequences. (Reannouncement with New Availability Information). [R] . Bell, J., Ratner, L. 1989

机译：人类免疫缺陷病毒1型DNa序列的聚合酶链扩增反应的特异性。（重新公布新的可用性信息）。

Sequence specificity of triplex DNA formation: Analysis by a combinatorial approach, restriction endonuclease protection selection and amplification

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