In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities

Takashi Sera; Peter G. Schultz

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In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities

机译：DNA结合特异性改变的基本区域-亮氨酸拉链蛋白的体内选择

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A transcription interference assay was used to generate mutant basic region-leucine zipper proteins with altered DNA-binding specificities. A library of mutants of a CCAAT/enhancer binding protein was constructed by ran- domizing five DNA-contacting amino acids in the basic region Asn~-18, Ala~-15, Val~-14, Ser~-11, and Arg~-10.

机译：转录干扰测定法用于产生具有改变的DNA结合特异性的突变基本区域-亮氨酸拉链蛋白。通过随机分配基本区域Asn〜-18，Ala〜-15，Val〜-14，Ser〜-11和Arg〜中的五个与DNA接触的氨基酸，构建了CCAAT /增强子结合蛋白的突变体文库。 -10。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第7期|p.2920-2925|共6页
作者
Takashi Sera; Peter G. Schultz;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Do basic region-leucine zipper proteins bend their DNA targets ... does it matter? [J] . Paul J. Hagerman Proceedings of the National Academy of Sciences of the United States of America . 1996,第19期

机译：碱性区域亮氨酸拉链蛋白会弯曲它们的DNA靶点吗？有关系吗？
2. DNA-binding specificity of the PAR basic leucine zipper protein VBP partially overlaps those of the C/EBP and CREB/ATF families and is influenced by domains that flank the core basic region. [J] . N B Haas, C A Cantwell, P F Johnson, Molecular and Cellular Biology . 1995,第4期

机译：PAR基本亮氨酸拉链蛋白VBP的DNA结合特异性与C / EBP和CREB / ATF家族的DNA结合特异性部分重叠，并受位于核心碱性区域侧翼的域的影响。
3. A novel DNA binding mechanism for maf basic region-leucine zipper factors inferred from a MafA-DNA complex structure and binding specificities [J] . Lu X., Guanga G.P., Wan C., Biochemistry . 2012,第48期

机译：从MafA-DNA复合物结构和结合特异性推断出的Maf基本区域-亮氨酸拉链因子的新型DNA结合机制
4. Single-chain 434 repressors with altered DNA-binding specificities [C] . NATO advanced research workshop on structural biology and functional genomics . 1999

机译：具有改变的DNA结合特异性的单链434阻遏物
5. THE RESTRICTION ENDONUCLEASE ECO RI: ITS INTRINSIC METAL CENTER AND SPECIFIC INTERACTIONS WITH A DNA RECOGNITION SITE (PROTEIN-NUCLEIC ACID, ZINC METALLOENZYMES, INTERACTIONS CHIRAL METAL COMPLEXES, DNA-BINDING PROTEINS). [D] . PARANAWITHANA, SHANTHI R. 1986

机译：限制性内切酶Eco RI：其内在金属中心以及与DNA识别位点的特定相互作用（蛋白-核酸，锌金属酶，相互作用的手性金属络合物，DNA结合蛋白）。
6. In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities. [O] . T Sera, P G Schultz 1996

机译：具有改变的DNA结合特异性的基本区域-亮氨酸拉链蛋白的体内选择。
7. In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities. [O] . Sera, T, Schultz, P G 1996

机译：具有改变的DNA结合特异性的基本区域-亮氨酸拉链蛋白的体内选择。

In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities

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