Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases

Mark Brenneman; Frederick S. Gimble; John H. Wilson

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Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases

机译：通过位点特异性核酸内切酶的电穿孔刺激人类细胞中的染色体内同源重组

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In somatic mammalian cells, homologous recombination is a rare event. To study the effects of chro- mosomal breaks on frequency of homologous recombination, site-specific endonucleases were introduced into human cells by electroporation. Cell lines with a partial duplication within the HPRT (hypoxanthine phosphoribosyltransferase) gene were created through gene targeting. Homologous, intrachro- mosomal recombination between the repeated regions of the gene can reconstruct a functioning, wild-type gene.

机译：在体细胞哺乳动物细胞中，同源重组是罕见的事件。为了研究染色体断裂对同源重组频率的影响，通过电穿孔将位点特异性核酸内切酶引入人细胞。通过基因靶向，建立了HPRT（次黄嘌呤磷酸核糖基转移酶）基因内部分重复的细胞系。基因重复区域之间的同源染色体内重组可以重建功能性野生型基因。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第8期|p.3608-3612|共5页
作者
Mark Brenneman; Frederick S. Gimble; John H. Wilson;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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专利

1. Analysis of Gene Targeting and Intrachromosomal Homologous Recombination Stimulated by Genomic Double-Strand Breaks in Mouse Embryonic Stem Cells [J] . Greg Donoho, Maria Jasin, Paul Berg Molecular and Cellular Biology . 1998,第7期

机译：基因组双链断裂在小鼠胚胎干细胞中刺激基因靶向和染色体内同源重组的分析
2. Stimulation of intrachromosomal homologous recombination in mammalian cells by ain inhibitor of poly(ADP-ribosylation) [J] . Alan S. Waldman, Barbara Criscuolo Waldman Nucleic acids research . 1991,第21期

机译：聚（ADP-核糖基化）的ain抑制剂刺激哺乳动物细胞中的染色体内同源重组
3. Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease [J] . Barbara Hohn, Bernard Dujon, Holger Puchta Nucleic acids research . 1993,第22期

机译：通过位点特异性核酸内切酶在体内诱导双链断裂成DNA来增强植物细胞中的同源重组
4. TARGETED DISRUPTION OF alpha-1, 6-FUCOSYLTRANSFERASE (FUT8) GENE BY HOMOLOGOUS RECOMBINATION IN CHINESE HAMSTER OVARY (CHO) CELLS [C] . Masayoshi Tsukahara, Ayako Aoki, Keina Kozono, Annual Meeting of the Japanese Association for Animal Cell Technology . 2006

机译：通过在中国仓鼠卵巢（CHO）细胞中的同源重组来靶向破坏α-1,6-岩氧基转移酶（FUT8）基因的破坏
5. Characterization of intrachromosomal homologous recombination in mammalian cells. [D] . Ruksc, Anna. 2008

机译：哺乳动物细胞内染色体内同源重组的表征。
6. Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. [O] . M Brenneman, F S Gimble, J H Wilson 1996

机译：通过位点特异性核酸内切酶的电穿孔刺激人类细胞中的染色体内同源重组。
7. Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. [O] . Brenneman, M, Gimble, F S, Wilson, J H 1996

机译：通过位点特异性核酸内切酶的电穿孔刺激人类细胞中的染色体内同源重组。
8. Mutations Induced by 1-Nitrosopyrene and Related Compounds during DNA Replicationin Human Cells and Induction of Homologous Recombination by These Compounds [R] . Maher, V. M., Bhattacharyya, N. P., Mah, M. C. C., 1993

机译：人体细胞DNa复制过程中1-亚硝基芘和相关化合物诱导的突变及这些化合物同源重组的诱导

Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases

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