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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Movement of yeast cortical actin cytoskeleton visualized in vivo
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Movement of yeast cortical actin cytoskeleton visualized in vivo

机译:体内可视化酵母皮质肌动蛋白细胞骨架的运动

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摘要

Fusion proteins between the green fluores- cent protein (GFP) and the cytoskeleton proteins Act1p (actin), Sac6p (yeast fimbrin homolog), and Abp1p in budding yeast (Saccharomyces cerevisiae) localize to the cortical actin patches. The actin fusions could not function as the sole actin source in yeast, but fusions between the actin-binding proteins Abp1p and Sac6p complement fully the phenotypes associated with their gene deletions. Direct observation in vivo reveals that the actin cortical patches move.
机译:绿色荧光蛋白(GFP)与细胞骨架蛋白Act1p(肌动蛋白),Sac6p(酵母菌丝蛋白同系物)和Abp1p之间的融合蛋白位于发芽酵母(酿酒酵母)中,位于皮质肌动蛋白斑片上。肌动蛋白融合不能充当酵母中唯一的肌动蛋白来源,但是肌动蛋白结合蛋白Abp1p和Sac6p之间的融合完全补充了与它们的基因缺失相关的表型。体内直接观察发现肌动蛋白皮质斑块移动。

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