Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis

Barbara A. Bensing; Barbara J. Meyer; Gary M. Dunny

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis

【24h】

Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis

机译：信息素诱导的粪肠球菌质粒转移系统中细菌转录起始位点的敏感检测和RNA加工位点的分化

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A method was developed to detect 5' ends of bacterial RNAs expressed at low levels and to differentiate newly initiated transcripts from processed transcripts pro- duced in vivo. The procedure involves use of RNA ligase to link a specific oligoribonucleotide to the 5' ends of cellular RNAs, followed by production of cDNA and amplification of the gene of interest by PCR. The method was used to identify the precise sites of transcription initiation within a 10-kb region of the pheromone-inducible conjugative plasmid pCF10 of Entero- coccus faecalis.

机译：开发了一种方法来检测低水平表达的细菌RNA的5'末端，并将新启动的转录本与体内产生的加工转录本区分开。该过程包括使用RNA连接酶将特定的寡核糖核苷酸连接到细胞RNA的5'末端，然后产生cDNA，并通过PCR扩增目的基因。该方法用于鉴定粪肠球菌的信息素诱导型结合质粒pCF10的10-kb区域内转录起始的精确位点。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第15期|p.7794-7799|共6页
作者
Barbara A. Bensing; Barbara J. Meyer; Gary M. Dunny;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词

相似文献

外文文献
中文文献
专利

1. Peptide pheromone-induced transfer of plasmid pCF10 in Enterococcus faecalis: probing the genetic and molecular basis for specificity of the pheromone response. [J] . Dunny GM iosci.cbs.umn.edu, Antiporta MH, Hirt H Peptides: An International Journal . 2001,第10期

机译：肽信息素诱导的粪肠球菌质粒pCF10转移：探查信息素应答特异性的遗传和分子基础。
2. Replication of Enterococcus faecalis Pheromone-Responding Plasmid pAD1: Location of the Minimal Replicon and oriV Site and RepA Involvement in Initiation of Replication [J] . Maria Victoria Francia, Shuhei Fujimoto, Patricia Tille, Journal of bacteriology . 2004,第15期

机译：粪肠球菌信息素响应质粒pAD1的复制：最小复制子和oriV位点的位置以及RepA参与复制的启动
3. Transfer origins in the conjugative Enterococcus faecalis plasmids pAD1 and pAM373: identification of the pAD1 nic site, a specific relaxase and a possible TraG-like protein. [J] . Francia MV, Clewell DB Molecular Microbiology . 2002,第2期

机译：转移性粪肠球菌质粒pAD1和pAM373的起源：pAD1 nic位点，特异性松弛酶和可能的TraG样蛋白的鉴定。
4. ELECTRON MICROSCOPIC INVESTIGATION OF THE SITE OF ANTIMICROBIAL ACTIVITY OF HENTRIACONTANE ON Enterococcus faecalis [C] . F. S. Botha, I. L. Elisha, A. R. Jambalang, Annual Conference of the Microscopy Society of Southern Africa . 2017

机译：电子显微镜调查肠球菌粪便中的抗菌活性抗菌活性遗址
5. Genetic and transcriptional analyses of the pheromone response of the Enterococcus faecalis plasmid pAM373. [D] . De Boever, Erika Helena. 2001

机译：粪肠球菌质粒pAM373信息素应答的遗传和转录分析。
6. Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis. [O] . B A Bensing, B J Meyer, G M Dunny 1996

机译：敏感检测细菌转录起始位点并从信息素诱导的粪肠球菌质粒转移系统中的RNA加工位点分化。
7. Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis. [O] . Bensing, B A, Meyer, B J, Dunny, G M 1996

机译：敏感检测细菌转录起始位点，并从信息素诱导的粪肠球菌质粒转移系统中的RNA加工位点分化。
8. RNA Polymerase-Binding and Transcription Initiation Sites Upstream of the Methyl Reductase Operon of 'Methanococcus vannielii' [R] . Thomm, M., Sherf, B. A., Reeve, J. N. 1988

机译：RNa聚合酶结合和转录起始位点'methanococcus vannielii'的甲基还原酶操纵子的上游

Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis

摘要

著录项

相似文献

相关主题

期刊订阅