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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Association of yeast SIN1 with the tetratrico peptide repeats of CDC23
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Association of yeast SIN1 with the tetratrico peptide repeats of CDC23

机译:酵母SIN1与CDC23的四肽重复序列的关联

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摘要

The yeast SIN1 protein is a nuclear protein that together with other proteins behaves as a transcriptional repressor of a family of genes. In addition, sin1 mutants are defective in proper mitotic chromosome segregation. In an effort to understand the basis for these phenotypes, we employed the yeast two-hybrid system to identify proteins that interact with SIN1 in vivo. Here we demonstrate that CDC23, a protein known to be involved in sister chromatid separation during mitosis, is able to directly interact with SIN1.
机译:酵母SIN1蛋白是一种核蛋白,与其他蛋白一起充当基因家族的转录阻遏物。此外,sin1突变体在适当的有丝分裂染色体分离方面存在缺陷。为了理解这些表型的基础,我们采用了酵母双杂交系统来鉴定与SIN1在体内相互作用的蛋白质。在这里,我们证明CDC23是一种已知与有丝分裂期间姐妹染色单体分离有关的蛋白质,它能够与SIN1直接相互作用。

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