Methylation of coding region alone inhibits gene expression in plant protoplasts

Thomas Hohn; Sandra Corsten; Silke Rieke

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Methylation of coding region alone inhibits gene expression in plant protoplasts

机译：单独的编码区的甲基化抑制植物原生质体中的基因表达

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Derivatives of the cauliflower mosaic virus 35S promoter lacking CG and CNG methylation targets were constructed and used to direct transcription of reporter gene constructs in transiently transformed protoplasts. Such methylation-target-free (MTF) promoters, although weaker than the 35S promoter, retain significant activity despite mutation of the as-1 element. The effect of methylation on gene expression in MTF- and 35S-promoter driven constructs was examined.

机译：构建了缺少CG和CNG甲基化靶标的花椰菜花叶病毒35S启动子的衍生物，并将其用于指导瞬时转化的原生质体中报道基因构建体的转录。这样的无甲基化靶标（MTF）启动子尽管比35S启动子弱，但尽管as-1元素发生了突变，仍保留了显着的活性。检验了甲基化对MTF和35S启动子驱动的构建体中基因表达的影响。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第16期|p.8334-8339|共6页
作者
Thomas Hohn; Sandra Corsten; Silke Rieke;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Gene transfer in plant protoplasts Inhibition of gene activity by cytosine methylation and expression of single‐stranded DNA constructs [J] . Gruenbaum Yosef, Hershkovitz Maty, Loyter Abraham, FEBS Letters . 1989,第1a2期

机译：植物原生质体中的基因转移通过胞嘧啶甲基化抑制基因活性和单链DNA构建体的表达
2. Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector [J] . Ingo Potrykus, Maciej Pietrzak, Raymond D. Shillito, Nucleic acids research . 1986,第14期

机译：新型植物表达载体原生质体转化后两个细菌抗药性基因在植物中的表达
3. Molecular analysis of a U3 RNA gene locus in tomato: transcription signals, the coding region, expression in transgenic tobacco plants and tandemly repeated pseudogenes [J] . Ferenc Solymosy, Tamás Kiss Nucleic acids research . 1990,第8期

机译：番茄U3 RNA基因位点的分子分析：转录信号，编码区，转基因烟草植物中的表达以及串联重复的假基因
4. TRANSIENT EXPRESSION OF GENES WITH ALTERED METHYLATION LEVELS IN TOBACCO PROTOPLASTS [C] . N. BUCHERNA, L.E. HESZKY, I. NAGY Induced mutations and molecular techniques for crop improvement . 1995

机译：甲基化水平改变的基因在烟草原生质体中的瞬时表达
5. Regulation of human cytomegalovirus gene expression by changes in histone H3 acetylation and methylation, and through binding of transcription factors to promoter regions during replication in fibroblasts. [D] . Cuevas, Christian David. 2009

机译：通过组蛋白H3乙酰化和甲基化的变化以及成纤维细胞复制过程中转录因子与启动子区域的结合来调节人类巨细胞病毒基因表达。
6. Methylation of coding region alone inhibits gene expression in plant protoplasts. [O] . T Hohn, S Corsten, S Rieke, 1996

机译：单独的编码区的甲基化抑制植物原生质体中的基因表达。
7. Methylation of coding region alone inhibits gene expression in plant protoplasts. [O] . Hohn, T, Corsten, S, Rieke, S, 1996

机译：单独的编码区的甲基化抑制植物原生质体中的基因表达。

Methylation of coding region alone inhibits gene expression in plant protoplasts

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