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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The yeast GAL11 protein binds to the transcription factor IIE through GAL11 regions essential for its in vivo function
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The yeast GAL11 protein binds to the transcription factor IIE through GAL11 regions essential for its in vivo function

机译:酵母GAL11蛋白通过其体内功能必不可少的GAL11区域与转录因子IIE结合

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摘要

The GAL11 gene encodes an auxiliary tran- scription factor required for full expression of many genes in yeast. The GAL11-encoded protein (Gal11p) has recently been shown to copurify with the holoenzyme of RNA polymerase II. Here we report that Gal11p stimulates basal transcription in a reconstituted transcription system composed of recombi- nant or highly purified transcription factors, TFIIB, TFIIE, TFIIF, TFIIH, and TATA box-binding protein and core RNA polymerase II.
机译:GAL11基因编码酵母中许多基因充分表达所需的辅助转录因子。最近显示,GAL11编码的蛋白(Gal11p)与RNA聚合酶II的全酶共纯化。在这里,我们报道Gal11p在重组或高度纯化的转录因子,TFIIB,TFIIE,TFIIF,TFIIH和TATA盒结合蛋白以及核心RNA聚合酶II组成的重组转录系统中刺激基础转录。

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