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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The entry and clearance of Ca~2+ at individual presynaptic active zones of hair cells from the bullfrog's sacculus
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The entry and clearance of Ca~2+ at individual presynaptic active zones of hair cells from the bullfrog's sacculus

机译:Ca〜2 +在牛蛙s突毛细胞个别突触前活动区的进入和清除

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摘要

Neurotransmitter is released when Ca~2+ triggers the fusion of synaptic vesicles with the plasmalemma. To study factors that regulate Ca~2+ concentration at the presynaptic active zones of hair cells, we used laser-scanning confocal microscopy with the fluorescent Ca~2+ indicator fluo-3. The experimental results were compared with the predictions of a model of presynaptic Ca~2+ concentration in which Ca~2+ enters a cell through a point source, diffuses from the entry site, and binds to fixed or mobile Ca~2+ buffers.
机译:当Ca〜2 +触发突触小泡与质膜融合时,神经递质被释放。为了研究调节毛细胞突触前活性区Ca〜2 +浓度的因素,我们使用了带有荧光Ca〜2 +指示剂fluo-3的激光扫描共聚焦显微镜。将实验结果与突触前Ca〜2 +浓度模型的预测结果进行比较,在该模型中,Ca〜2 +通过点源进入细胞,从进入部位扩散,并与固定或移动的Ca〜2 +缓冲液结合。

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