Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells

Hagit Eldar-Finkelman; Gretchen M. Argast; Orit Foord

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Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells

机译：糖原合酶激酶3突变体的表达，表征及其对完整细胞糖原合酶活性的影响

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In these studies we expressed and character- ized wild-type (WT) GSK-3 (glycogen synthase kinase-3) and its mutants, and examined their physiological effect on gly- cogen synthase activity. The GSK-3 mutants included muta- tion at serine-9 either to alanine (S9A) or glutamic acid (S9E) and an inactive mutant, K85,86MA. Expression of WT and the various mutants in a cell-free system indicated that S9A and S9E exhibit increased kinase activity as compared with WT.

机译：在这些研究中，我们表达并表征了野生型（WT）GSK-3（糖原合酶激酶-3）及其突变体，并研究了它们对糖原合酶活性的生理影响。 GSK-3突变体包括在丝氨酸9处突变为丙氨酸（S9A）或谷氨酸（S9E），以及一个无活性的突变体K85,86MA。 WT和各种突变体在无细胞系统中的表达表明，与WT相比，S9A和S9E表现出增加的激酶活性。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第19期|p.10228-10233|共6页
作者
Hagit Eldar-Finkelman; Gretchen M. Argast; Orit Foord;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Glycogen Synthase Kinase-3 beta 2 Has Lower Phosphorylation Activity to Tau than Glycogen Synthase Kinase-3 beta 1 [J] . Saeki K, Machida M, Kinoshita Y, Biological & pharmaceutical bulletin . 2011,第1期

机译：糖原合酶激酶3 beta 2比糖原合酶激酶3 beta 1具有更低的磷酸化Tau活性。
2. The effects of glucose and the hexosamine biosynthesis pathway on glycogen synthase kinase-3 and other protein kinases that regulate glycogen synthase activity. [J] . Singh LP, Crook ED Journal of investigative medicine . 2000,第4期

机译：葡萄糖和己糖胺生物合成途径对糖原合酶激酶3和其他调节糖原合酶活性的蛋白激酶的影响。
3. The effects of glucose and the hexosamine biosynthesis pathway on glycogen synthase kinase-3 and other protein kinases that regulate glycogen synthase activity. [J] . Singh LP, Crook ED Journal of investigative medicine . 2000,第4期

机译：葡萄糖和己糖胺生物合成途径对糖原合酶激酶3和其他调节糖原合酶活性的蛋白激酶的影响。
4. Fuzzy Handling of Uncertainties in Modeling the Inhibition of Glycogen Synthase Kinase-3 by Paullones [C] . Kumar, Mohit, Thurow, . 2007

机译：Paullones对糖原合酶激酶3抑制作用建模不确定性的模糊处理
5. Glycogen Synthase Kinase-3 Loss-Of-Function Studies In Mus musculus and Murine Embryonic Stem Cells. [D] . Popkie, Anthony. 2011

机译：在小家鼠和小鼠胚胎干细胞中糖原合酶激酶3的功能丧失研究。
6. Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells. [O] . H Eldar-Finkelman, G M Argast, O Foord, 1996

机译：糖原合酶激酶3突变体的表达和表征及其对完整细胞中糖原合酶活性的影响。
7. Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells. [O] . Eldar-Finkelman, H, Argast, G M, Foord, O, 1996

机译：糖原合酶激酶3突变体的表达和表征及其对完整细胞中糖原合酶活性的影响。

Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells

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