Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: Application to the enzymatic formation of cholesta-5,8-dien-3β-ol

Benfang Ruan; Justin Shey; Nicolas Gerst

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: Application to the enzymatic formation of cholesta-5,8-dien-3β-ol

【24h】

Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: Application to the enzymatic formation of cholesta-5,8-dien-3β-ol

机译：银离子高压液相色谱提供前所未有的固醇分离：在酶促形成胆甾醇5,8-dien-3β-ol中的应用

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We report that silver ion HPLC provides remarkable separations of C_27 sterols differing only in the number or location of olefinic double bonds. This technique has been extended to LC-MS, analysis of purified components by GC, GC-MS, and ~1H NMR, and to its use on a semiprepara- tive scale. The application of this methodology for the dem- onstration of the catalysis, by rat liver microsomes, of the conversion of 7-dehydrocholesterol to cholesta-5,8-dien-3-ol is also presented.

机译：我们报道银离子HPLC提供了C_27固醇的显着分离，仅在烯烃双键的数量或位置上不同。这项技术已扩展到LC-MS，通过GC，GC-MS和〜1H NMR分析纯化的成分，并扩展到半制备规模。还介绍了该方法在证明大鼠肝微粒体催化7-脱氢胆固醇向cholesta-5,8-dien-3-ol转化中的应用。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第21期|p.11603-11608|共6页
作者
Benfang Ruan; Justin Shey; Nicolas Gerst;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词

相似文献

外文文献
中文文献
专利

1. STEROL SYNTHESIS - A TIMELY LOOK AT THE CAPABILITIES OF CONVENTIONAL AND SILVER ION HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE SEPARATION OF C-27 STEROLS RELATED TO CHOLESTEROL BIOSYNTHESIS [J] . Ruan BF., Emmons GT., Shey J., Journal of Lipid Research . 1997,第12期

机译：固醇合成-及时发现常规和银离子高效液相色谱法用于分离与胆固醇生物合成有关的C-27固醇的能力
2. Fast Separation and Determination of Sterols in Vegetable Oils by Ultraperformance Liquid Chromatography with Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection [J] . Maria Jesus Lerma-Garcia, Ernesto Francisco Simo-Alfonso, Alberto Mendez Journal of Agricultural and Food Chemistry . 2010,第5期

机译：超高效液相色谱-常压化学电离质谱法快速分离测定植物油中的甾醇
3. Preparative separation of α- And β-santalenes and (Z)-α- and (Z)-β-santalols using silver nitrate-impregnated silica gel medium pressure liquid chromatography and analysis of sandalwood oil [J] . Daramwar P.P., Srivastava P.L., Priyadarshini B., The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2012,第19期

机译：硝酸银浸渍的硅胶中压液相色谱法分离制备檀香油中的α-，β-桑塔龙，（Z）-α-和（Z）-β-檀香醇
4. Liquid chromatography with substrate-assisted laser desorption/ionization mass spectrometry for determination of sterols [C] . Blanka Vrbkova, Jan Preisler, Vendula Roblova, International Mass Spectrometry Conference . 2014

机译：具有基板辅助激光解吸/电离质谱法的液相色谱法测定甾醇
5. Development and application of gradient ultrahigh pressure liquid chromatography for separations of complex biological mixtures [D] . Link, Jason C. 2004

机译：梯度超高压液相色谱法分离复杂生物混合物的开发与应用
6. Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-58-dien-3 beta-ol. [O] . B Ruan, J Shey, N Gerst, 1996

机译：银离子高压液相色谱提供了空前的固醇分离：应用于酶促形成cholesta-58-dien-3β-ol。
7. Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-5,8-dien-3 beta-ol. [O] . Ruan, B, Shey, J, Gerst, N, 1996

机译：银离子高压液相色谱提供了前所未有的固醇分离：应用于酶促形成胆甾醇5,8-dien-3β-ol。

Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: Application to the enzymatic formation of cholesta-5,8-dien-3β-ol

摘要

著录项

相似文献

相关主题

期刊订阅