Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells

Tao Cheng; Hongmei Shen; Dena Giokas

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Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells

机译：单个原代造血细胞分化过程中基因表达水平的时间作图

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A hierarchical order of gene expression has been proposed to control developmental events in hematopoiesis, but direct demonstration of the temporal relationships between regulatory gene expression and differentiation has been difficult to achieve. We modified a single-cell PCR method to detect 2-fold changes in mRNA copies per cell (dynamic range, 250-250,000 copies/cell) and used it to sequentially quantitate gene expres- sion levels as single primitive (CD34~+,CD38~-) progenitor cells underwent differentiation to become erythrocytes, granulocytes, or monocyte/macrophages.

机译：已经提出了基因表达的分级顺序来控制造血过程中的发育事件，但是调节基因表达和分化之间的时间关系的直接证明一直难以实现。我们修改了单细胞PCR方法，以检测每个细胞中mRNA拷贝的2倍变化（动态范围为250-250,000拷贝/细胞），并使用它来定量定量基因表达水平作为单个原始基因（CD34〜+，CD38 〜-）祖细胞经历分化而变成红细胞，粒细胞或单核细胞/巨噬细胞。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第23期|p.13158-13163|共6页
作者
Tao Cheng; Hongmei Shen; Dena Giokas;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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6. Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells [O] . Tao Cheng, Hongmei Shen, Dena Giokas, 1996

机译：基因表达水平的时间映射单个原代造血细胞的分化
7. Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells [O] . T. Cheng, H. Shen, D. Giokas, 1996

机译：单个原发性造血细胞分化期间基因表达水平的时间映射

Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells

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