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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Analysis of the human guanylin gene and the processing and cellular localization of the peptide.
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Analysis of the human guanylin gene and the processing and cellular localization of the peptide.

机译：人鸟苷蛋白基因的分析以及该肽的加工和细胞定位。

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摘要

The complete cell biological analysis of human guanylin, a recently discovered regulatory peptide, is offered in this investigation: (i) the nucleotide sequence of the gene, (ii) the isolation and characterization of its circulating molecular form, and (iii) its localization in enterochromaffin cells of the gut. As determined by molecular cloning, DNA sequencing, and comparison with the known cDNA sequence, the approximately 2.6-kbp large gene consists of three exons interrupted by two introns. The putative promoter region contains a TTTAAAA sequence motif and several potential binding sites for transcription factors such as AP-1, AP-2, Sp 1, and glucocorticoid receptors. The isolated hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3 kDa. Western blot analysis of RP-HPLC fractions from blood plasma confirms this molecular form. Thus, guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood.
机译：这项研究提供了对人类鸟苷蛋白（一种最近发现的调节肽）的完整细胞生物学分析：（i）基因的核苷酸序列，（ii）其循环分子形式的分离和表征，以及（iii）其定位在肠道的肠嗜铬细胞中。通过分子克隆，DNA测序以及与已知cDNA序列的比较确定，大约2.6kbp的大基因由被两个内含子打断的三个外显子组成。推定的启动子区域包含TTTAAAA序列基序和转录因子（例如AP-1，AP-2，Sp 1和糖皮质激素受体）的几个潜在结合位点。鸟苷蛋白的分离的激素形式是分子量为10.3kDa的94个氨基酸的肽。血浆中RP-HPLC组分的蛋白质印迹分析证实了这种分子形式。因此，鸟苷蛋白是由肠肠嗜铬细胞作为115个氨基酸的激素激素合成的，并被加工成血液中循环的94个氨基酸的分子形式。

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