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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Increased sensitivity to γ irradiation in bacteria lacking protein HU
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Increased sensitivity to γ irradiation in bacteria lacking protein HU

机译：缺乏蛋白HU的细菌对γ辐射的敏感性增加

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摘要

The heterodimeric HU protein, isolated from Escherichia coli, is associated with the bacterial nucleoid and shares some properties with both histones and HMG proteins. It is the prototype of small bacterial DNA binding proteins with a pleiotropic role in the cell. HU participates in several biological processes like cell division, initiation of DNA replication, transposition, and other biochemical functions. We show here that bacteria lacking HU are extremely sensitive to γ irradiation. Expression of either one of the subunits of HU in the hupAB double mutant nearly restores the normal survival rate. This shows that the sensitivity is due to the absence of HU rather than being the result of a secondary mutation occurring in the hupAB cells or a modification of the SOS repair system, since SOS genes are induced normally in the absence of HU. Finally, in vitro studies give an indication of its potential role: HU protects DNA against cleavage by γ-rays.
机译：分离自大肠杆菌的异二聚体HU蛋白与细菌核苷有关，并与组蛋白和HMG蛋白具有某些特性。它是在细胞中具有多效性作用的小细菌DNA结合蛋白的原型。 HU参与了多个生物学过程，例如细胞分裂，DNA复制的起始，转座和其他生化功能。我们在这里表明缺乏HU的细菌对γ辐射极为敏感。在hupAB双突变体中HU的任一亚基的表达几乎恢复了正常的存活率。这表明敏感性是由于不存在HU引起的，而不是由于hupAB细胞中发生二次突变或修饰了SOS修复系统的结果，因为SOS基因通常在不存在HU的情况下被诱导。最后，体外研究表明了其潜在作用：HU保护DNA免受γ射线切割。

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