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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana.
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A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana.

机译：拟南芥中的脱水和盐胁迫诱导了编码磷脂酰肌醇特异性磷脂酶C的基因。

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摘要

A cDNA corresponding to a putative phosphatidylinositol-specific phospholipase C (PI-PLC) in the higher plant Arabidopsis thaliana was cloned by use of the polymerase chain reaction. The cDNA, designated cAtPLC1, encodes a putative polypeptide of 561 aa with a calculated molecular mass of 64 kDa. The putative product includes so-called X and Y domains found in all PI-PLCs identified to date. In mammalian cells, there are three types of PI-PLC, PLC-beta, -gamma, and -delta. The overall structure of the putative AtPLC1 protein is most similar to that of PLC-delta, although the AtPLC1 protein is much smaller than PLCs from other organisms. The recombinant AtPLC1 protein synthesized in Escherichia coli was able to hydrolyze phosphatidylinositol 4,5-bisphosphate and this activity was completely dependent on Ca2+, as observed also for mammalian PI-PLCs. These results suggest that the AtPLC1 gene encodes a genuine PI-PLC of a higher plant. Northern blot analysis showed that the AtPLC1 gene is expressed at very low levels in the plant under normal conditions but is induced to a significant extent under various environmental stresses, such as dehydration, salinity, and low temperature. These observations suggest that AtPLC1 might be involved in the signal-transduction pathways of environmental stresses and that an increase in the level of AtPLC1 might amplify the signal, in a manner that contributes to the adaptation of the plant to these stresses.
机译：利用聚合酶链反应，克隆了与拟南芥拟南芥中特定的磷脂酰肌醇特异性磷脂酶C（PI-PLC）相对应的cDNA。名为cAtPLC1的cDNA编码561个氨基酸的推定多肽，计算分子量为64 kDa。推定的产品包括迄今确定的所有PI-PLC中都存在的所谓X和Y域。在哺乳动物细胞中，存在三种类型的PI-PLC，PLC-beta，-gamma和-delta。假定的AtPLC1蛋白的总体结构与PLC-delta的最相似，尽管AtPLC1蛋白比其他生物体的PLC小得多。在大肠杆菌中合成的重组AtPLC1蛋白能够水解磷脂酰肌醇4,5-双磷酸酯，这种活性完全取决于Ca2 +，这在哺乳动物PI-PLC中也观察到。这些结果表明AtPLC1基因编码一个高等植物的真正的PI-PLC。 Northern印迹分析表明，AtPLC1基因在正常条件下在植物中的表达水平非常低，但是在各种环境胁迫下，例如脱水，盐度和低温下，AtPLC1基因的表达水平却很高。这些观察结果表明，AtPLC1可能参与了环境胁迫的信号转导途径，并且AtPLC1水平的升高可能以有助于植物适应这些胁迫的方式放大了信号。

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