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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Use of Arabidopsis thaliana defense-related mutants to dissect the plant response to pathogens.
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Use of Arabidopsis thaliana defense-related mutants to dissect the plant response to pathogens.

机译：拟南芥防御相关突变体用于解剖植物对病原体的反应。

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摘要

The plant defense response to microbial pathogens had been studied primarily by using biochemical and physiological techniques. Recently, several laboratories have developed a variety of pathosystems utilizing Arabidopsis thaliana as a model host so that genetic analysis could also be used to study plant defense responses. Utilizing a pathosystem that involves the infection of Arabidopsis with pathogenic pseudomonads, we have cloned the Arabidopsis disease-resistance gene RPS2, which corresponds to the avirulence gene avrRpt2 in a gene-for-gene relationship. RPS2 encodes a 105-kDa protein containing a leucine zipper, a nucleotide binding site, and 14 imperfect leucine-rich repeats. The RPS2 protein is remarkably similar to the product of the tobacco N gene, which confers resistance to tobacco mosaic virus. We have also isolated a series of Arabidopsis mutants that synthesize decreased levels of an Arabidopsis phytoalexin called camalexin. Analysis of these mutants indicated that camalexin does not playa significant role in limiting growth of avirulent Pseudomonas syringae strains during the hypersensitive defense response but that it may play a role in limiting the growth of virulent strains. More generally, we have shown that we can utilize Arabidopsis to systematically dissect the defense response by isolation and characterization of appropriate defense-related mutants.
机译：主要通过使用生物化学和生理学技术研究了植物对微生物病原体的防御反应。最近，几个实验室已经开发了多种利用拟南芥作为模型宿主的病理系统，因此遗传分析也可以用于研究植物防御反应。利用涉及致病性拟单胞菌感染拟南芥的病理系统，我们克隆了拟南芥抗病基因RPS2，该基因对应于无毒基因avrRpt2。 RPS2编码一个105-kDa的蛋白质，其中包含一个亮氨酸拉链，一个核苷酸结合位点和14个不完整的富含亮氨酸的重复序列。 RPS2蛋白与烟草N基因的产物非常相似，赋予了对烟草花叶病毒的抗性。我们还分离了一系列拟南芥植物抗生素，称为camalexin的合成水平下降的一系列拟南芥突变体。对这些突变体的分析表明，camalexin在超敏防御反应过程中对限制无毒丁香假单胞菌菌株的生长没有显著作用，但可能在限制有毒菌株的生长中起作用。更一般地说，我们已经表明我们可以利用拟南芥通过适当的防御相关突变体的分离和表征来系统地解剖防御反应。

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