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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Exit from G0 and entry into the cell cycle of cells expressing p21Sdi1 antisense RNA.
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Exit from G0 and entry into the cell cycle of cells expressing p21Sdi1 antisense RNA.

机译：从G0退出，进入表达p21Sdi1反义RNA的细胞的细胞周期。

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摘要

p21Sdi1 (also known as Cip1 and Waf1), an inhibitor of DNA synthesis cloned from senescent human fibroblasts, is an inhibitor of G1 cyclin-dependent kinases (Cdks) in vitro and is transcriptionally regulated by wild-type p53. In addition, p21Sdi1 has been found to inhibit DNA replication by direct interaction with proliferating cell nuclear antigen. In this study we analyzed normal human fibroblast cells arrested in G0 and determined that an excess of p21Sdi1 was present after immunodepletion of various cyclins and Cdks, in contrast to mitogen-stimulated cells in early S phase. Expression of antisense p21Sdi1 RNA in G0-arrested cells resulted in induction of DNA synthesis as well as entry into mitosis. These results suggest that p21Sdi1 functions in G0 and early G1 and that decreased expression of the gene is necessary for cell cycle progression.
机译：p21Sdi1（也称为Cip1和Waf1）是从衰老的人类成纤维细胞克隆的DNA合成抑制剂，在体外是G1细胞周期蛋白依赖性激酶（Cdks）的抑制剂，并受野生型p53转录调控。另外，已发现p21Sdi1通过与增殖细胞核抗原直接相互作用抑制DNA复制。在这项研究中，我们分析了在G0中停滞的正常人成纤维细胞，并确定了各种细胞周期蛋白和Cdks的免疫耗竭后存在过量的p21Sdi1，这与早期S期的促细胞分裂剂刺激的细胞相反。反义p21Sdi1 RNA在G0阻滞细胞中的表达导致DNA合成的诱导以及进入有丝分裂。这些结果表明，p21Sdi1在G0和早期G1中起作用，并且该基因表达的降低对于细胞周期进程是必需的。

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