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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Immunolocalization of the mercurial-insensitive water channel and glycerol intrinsic protein in epithelial cell plasma membranes.
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Immunolocalization of the mercurial-insensitive water channel and glycerol intrinsic protein in epithelial cell plasma membranes.

机译：汞不敏感的水通道和甘油固有蛋白在上皮细胞质膜中的免疫定位。

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摘要

Two water channel homologs were cloned recently from rat kidney, mercurial-insensitive water channel (MIWC) and glycerol intrinsic protein (GLIP). Polyclonal antibodies were raised against synthetic C-terminal peptides and purified by affinity chromatography. MIWC and GLIP antibodies recognized proteins in rat kidney with an apparent molecular mass of 30 and 27 kDa, respectively, and did not cross-react. By immunofluorescence, MIWC and GLIP were expressed together on the basolateral plasma membrane of collecting duct principal cells in kidney. By immunohistochemistry, MIWC and GLIP were expressed on tracheal epithelial cells with greater expression of GLIP on the basal plasma membrane and MIWC on the lateral membrane; only MIWC was expressed in bronchial epithelia. In eye, GLIP was expressed in conjunctival epithelium, whereas MIWC was found in iris, ciliary body, and neural cell layers in retina. MIWC and GLIP colocalized on the basolateral membrane of villus epithelial cells in colon and brain ependymal cells. Expression of MIWC and GLIP was not detected in small intestine, liver, spleen, endothelia, and cells that express water channels CHIP28 or WCH-CD. These studies suggest water/solute transporting roles for MIWC and GLIP in the urinary concentrating mechanism, cerebrospinal fluid absorption, ocular fluid balance, fecal dehydration, and airway humidification. The unexpected membrane colocalization of MIWC and GLIP in several tissues suggests an interaction at the molecular and/or functional levels.
机译：最近从大鼠肾脏中克隆了两个水通道同源物，对水uri不敏感的水通道（MIWC）和甘油固有蛋白（GLIP）。产生针对合成C末端肽的多克隆抗体，并通过亲和色谱法纯化。 MIWC和GLIP抗体识别大鼠肾脏中的蛋白质，其表观分子量分别为30和27 kDa，并且没有交叉反应。通过免疫荧光，MIWC和GLIP在肾脏中收集导管主细胞的基底外侧质膜上一起表达。通过免疫组织化学，MIWC和GLIP在气管上皮细胞中表达，GLIP在基底质膜上的表达更高，而MIWC在侧膜上的表达更高。在支气管上皮中仅表达MIWC。在眼中，GLIP在结膜上皮中表达，而MIWC在虹膜，睫状体和视网膜的神经细胞层中发现。 MIWC和GLIP共定位在结肠和脑室管膜细胞的绒毛上皮细胞的基底外侧膜上。在小肠，肝脏，脾脏，内皮和表达水通道CHIP28或WCH-CD的细胞中未检测到MIWC和GLIP的表达。这些研究表明MIWC和GLIP在尿液浓缩机制，脑脊液吸收，眼液平衡，粪便脱水和气道增湿中的水/溶质转运作用。 MIWC和GLIP在某些组织中的意外膜共定位表明了在分子和/或功能水平上的相互作用。

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