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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Incorporation of acetylcholine receptors and Cl- channels in Xenopus oocytes injected with Torpedo electroplaque membranes.
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Incorporation of acetylcholine receptors and Cl- channels in Xenopus oocytes injected with Torpedo electroplaque membranes.

机译：在用鱼雷电噬菌斑膜注射的非洲爪蟾卵母细胞中掺入乙酰胆碱受体和Cl-通道。

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摘要

A method was developed to transplant assembled nicotinic acetylcholine receptors (AcChoRs) and Cl- channels from the electric organ of Torpedo to the membrane of Xenopus oocytes. Membrane vesicles from Torpedo electroplaques were injected into the oocytes and, within a few hours, the oocyte membrane acquired AcChoRs and Cl- channels. The mechanism of expression of these receptors and channels is very different from that which follows the injection of mRNA, since the appearance of receptors after membrane injection does not require de novo protein synthesis or N-glycosylation. This, and other controls, indicate that the foreign receptor-bearing membranes fuse with the oocyte membrane and cause the appearance of functional receptors and channels. All this makes the Xenopus oocyte an even more powerful tool for studies of the structure and function of membrane proteins.
机译：开发了一种将组装好的烟碱乙酰胆碱受体（AcChoRs）和Cl-通道从鱼雷的电器官移植到非洲爪蟾卵母细胞膜的方法。将鱼雷电噬菌斑的膜囊泡注入卵母细胞，并在几个小时内，卵母细胞膜获得AcChoRs和Cl-通道。这些受体和通道的表达机制与mRNA注射后的表达机制有很大不同，因为膜注射后受体的出现不需要从头蛋白质合成或N-糖基化。这和其他对照表明，带有外来受体的膜与卵母细胞膜融合并导致功能性受体和通道的出现。所有这些使非洲爪蟾卵母细胞成为研究膜蛋白结构和功能的更强大工具。

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