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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Presence of antibodies to different subunits of replication protein A in autoimmune sera.
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Presence of antibodies to different subunits of replication protein A in autoimmune sera.

机译：自身免疫血清中存在针对复制蛋白A不同亚基的抗体。

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摘要

A human cDNA expression library was used to investigate the nature of molecules recognized by serum from a patient with Sjogren syndrome that exhibits a mixed immunofluorescence pattern and reacts with multiple components on an immunoblot. The data demonstrated that this serum contains IgG antibodies specific for the 70- and 32-kDa subunits of replication protein A (RPA; RPA-70 and RPA-32, respectively), a highly conserved multisubunit DNA binding protein. Affinity purification of serum autoantibodies demonstrated a complete lack of cross-reactivity between RPA-70 and RPA-32, suggesting a direct participation of the native protein complex in the autoimmune response in this patient. Purified anti-RPA-70 and anti-RPA-32 antibodies labeled nuclear and cytoplasmic components in an immunofluorescence assay, suggesting that RPA is present in both cellular compartments. Additional sera from 55 patients with different autoimmune conditions were screened against purified RPA-70 and RPA-32 recombinant proteins. One of these 55 sera was positive and reacted with only RPA-32. Twenty sera from healthy control individuals did not react with RPA. These results show that RPA is a target for autoantibodies in human autoimmune diseases, although its precise frequency, occurrence in other autoimmune diseases, and pathological significance remain to be fully elucidated.
机译：人类cDNA表达文库用于研究Sjogren综合征患者的血清识别的分子的性质，该患者表现出混合的免疫荧光模式并与免疫印迹上的多种成分反应。数据表明，该血清含有对复制蛋白A（分别为RPA； RPA-70和RPA-32）的70-kDa亚基具有特异性的IgG抗体，该蛋白是高度保守的多亚基DNA结合蛋白。血清自身抗体的亲和纯化显示RPA-70和RPA-32之间完全缺乏交叉反应性，表明该患者体内天然蛋白复合物直接参与自身免疫反应。纯化的抗RPA-70和抗RPA-32抗体在免疫荧光测定中标记了核和细胞质成分，表明RPA存在于两个细胞区室中。针对55例具有不同自身免疫条件的患者的其他血清，针对纯化的RPA-70和RPA-32重组蛋白进行了筛选。这55个血清中有一个是阳性的，仅与RPA-32反应。来自健康对照个体的二十份血清未与RPA反应。这些结果表明，RPA是人类自身免疫性疾病自身抗体的靶标，尽管它的确切频率，在其他自身免疫性疾病中的发生率以及病理学意义仍有待充分阐明。

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