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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Isolation of small polarized bile duct units.
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Isolation of small polarized bile duct units.

机译：隔离小极化胆管单元。

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摘要

Fragments of small interlobular bile ducts averaging 20 microns in diameter can be isolated from rat liver. These isolated bile duct units form luminal spaces that are impermeant to dextran-40 and expand in size when cultured in 10 microM forskolin for 24-48 hr. Secretion is Cl- and HCO3- dependent and is stimulated by forskolin > dibutyryl cAMP > secretion but not by dideoxyforskolin, as assessed by video imaging techniques. Secretin stimulates Cl-/HCO3- exchange activity, and intraluminal pH increases after forskolin administration. These studies establish that small polarized physiologically intact interlobular bile ducts can be isolated from rat liver. These isolated bile duct units should be useful preparations for assessing the transport properties of small bile duct segments, which are the primary site of injury in cholestatic liver disorders, known as "vanishing bile duct syndromes."
机译：可以从大鼠肝脏中分离出平均直径为20微米的小叶间小胆管碎片。这些孤立的胆管单元形成了腔室空间，这些腔室对葡聚糖40而言是不渗透的，并且在10 microM福司柯林中培养24-48小时时尺寸会扩大。分泌是Cl-和HCO3-依赖性的，如视频成像技术所评估，其受到毛喉素>二丁酰基cAMP>分泌的刺激，但不受到双脱氧毛喉素的刺激。促胰液素可刺激Cl- / HCO3-交换活性，并且在施用毛喉素后腔内pH升高。这些研究证实，可以从大鼠肝脏中分离出小极化的生理上完整的小叶间胆管。这些孤立的胆管单元应该是有用的准备，用于评估小胆管段的运输特性，胆小段是胆汁淤积性肝病（称为“消失的胆管综合症”）的主要损伤部位。

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