...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >ISOLATION OF MULTIPLE SEQUENCES FROM THE PLASMODIUM FALCIPARUM GENOME THAT ENCODE CONSERVED DOMAINS HOMOLOGOUS TO THOSE IN ERYTHROCYTE-BINDING PROTEINS
【24h】

ISOLATION OF MULTIPLE SEQUENCES FROM THE PLASMODIUM FALCIPARUM GENOME THAT ENCODE CONSERVED DOMAINS HOMOLOGOUS TO THOSE IN ERYTHROCYTE-BINDING PROTEINS

机译：恶性疟原虫基因组中多个序列的编码

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Open reading frames in the Plasmodium falciparum genome encode domains homologous to the adhesive domains of the P. falciparum EBA-175 erythrocyte-binding protein (eba-175 gene product) and those of the Plasmodium vivax and Plasmodium knowlesi Duffy antigen-binding proteins. These domains are referred to as Duffy binding-like (DBL), after the receptor that determines P. vivax invasion of Duffy blood group-positive human erythrocytes. Using oligonucleotide primers derived from short regions of conserved sequence, we have developed a reverse transcription-PCR method that amplifies sequences encoding the DBL domains of expressed genes. Products of these reverse transcription-PCR amplifications include sequences of single-copy genes (including eba-175) and variably transcribed genes that cross-hybridize to multiple regions of the genome. Restriction patterns of the multicopy genes show a high degree of polymorphism among different parasite lines, whereas single-copy genes are generally conserved. Characterization of the single-copy genes has identified a gene (ebl-1) that is related to eba-175 and is likely to be involved in erythrocyte invasion. [References: 20]
机译：恶性疟原虫基因组中的开放阅读框编码与恶性疟原虫EBA-175红细胞结合蛋白（eba-175基因产物）以及间日疟原虫和诺氏疟原虫达菲抗原结合蛋白的粘附域同源的域。这些结构域在决定间日疟原虫入侵达菲血型阳性人红细胞的受体之后被称为达菲结合样（DBL）。使用衍生自保守序列短区的寡核苷酸引物，我们开发了一种逆转录-PCR方法，该方法可扩增编码表达基因的DBL域的序列。这些逆转录-PCR扩增产物包括单拷贝基因（包括eba-175）的序列和交叉杂交到基因组多个区域的可变转录基因。多拷贝基因的限制模式显示出不同寄生虫品系之间的高度多态性，而单拷贝基因通常是保守的。单拷贝基因的鉴定已经鉴定了与eba-175相关的基因（ebl-1），可能与红细胞的侵袭有关。 [参考：20]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号