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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >APPEARANCE OF INSULIN-LIKE GROWTH FACTOR MRNA IN THE LIVER AND PYLORIC CECA OF A TELEOST IN RESPONSE TO EXOGENOUS GROWTH HORMONE
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APPEARANCE OF INSULIN-LIKE GROWTH FACTOR MRNA IN THE LIVER AND PYLORIC CECA OF A TELEOST IN RESPONSE TO EXOGENOUS GROWTH HORMONE

机译：胰岛素样生长因子mRNA在外源性生长激素响应时的肝脏和食管癌性CECA中的出现

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Augmentation of vertebrate growth by growth hormone (GH) is primarily due to its regulation of insulin-like growth factor I (IGF I) and IGF II levels, To characterize the effect of GH on the levels of IGF I and IGF II mRNA in a teleost, 10 mu g of bovine GH (bGH) per g of body weight was administered to juvenile rainbow trout (Oncorhynchus mykiss) through i,p. injection. The levels of IGF I and IGF II mRNA were determined simultaneously, by using RNase protection assays, in the liver, pyloric ceca, kidney, and gill at 0, 1, 3, 6, 12, 24, 48, and 72 hr after injection, In the liver, IGF I mRNA levels were significantly elevated at 6 and 12 hr (approximate to 2- to 3-fold, P less than or equal to 0.01), while IGF II mRNA levels were significantly elevated at 3 and 6 hr (approximate to 3-fold, P less than or equal to 0.01), In the pyloric ceca, IGF II mRNA levels were significantly elevated at 12, 24, and 48 hr (approximate to 3-fold, P less than or equal to 0.01), while IGF I mRNA was below the limits of assay accuracy. GH-dependent IGF mRNA appearance was not detected in the gill and kidney, Serum bGH levels, determined by using a radioimmunoassay, were significantly elevated at 3 and 6 hr (P < 0.005), In primary hepatocyte culture, IGF I and IGF II mRNA levels increased in a bGH dose-dependent fashion, with ED(50) values of approximate to 45 and approximate to 6 ng of bGH per mi, respectively. The GH-dependent appearance of IGF II mRNA in the liver and pyloric ceca suggests important roles for this peptide hormone exclusive of IGF I. [References: 25]
机译：生长激素（GH）促进脊椎动物生长的主要原因是其对胰岛素样生长因子I（IGF I）和IGF II水平的调节，以表征GH对动物体内IGF I和IGF II mRNA水平的影响。硬骨鱼，通过幼虫对幼虹鳟（Oncorhynchus mykiss）施用每克体重10克牛生长激素（bGH）。注射。注射后0、1、3、6、12、24、48和72小时，通过RNase保护测定法同时测定肝脏，幽门盲肠，肾脏和腮中的IGF I和IGF II mRNA水平在肝脏中，IGF I mRNA水平在6和12小时显着升高（约2至3倍，P小于或等于0.01），而IGF II mRNA水平在3和6小时显着升高（约3倍，P小于或等于0.01），在幽门盲肠中，IGF II mRNA水平在第12、24和48小时显着升高（约3倍，P小于或等于0.01） ，而IGF I mRNA低于检测准确度的极限。在the和肾中未检测到GH依赖性IGF mRNA的出现，通过放射免疫测定法测定的血清bGH水平在3和6小时显着升高（P <0.005），在原代肝细胞培养中，IGF I和IGF II mRNA浓度以bGH剂量依赖性方式增加，ED（50）值分别约为每mi 45 bGH和6 ng bGH。肝脏和幽门盲肠中IGF II mRNA的GH依赖性出现表明该肽激素（IGF I除外）具有重要作用。[参考文献：25]

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