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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >IDENTIFICATION OF A SECOND HOMOLOG OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN THAT IS EXPRESSED IN THE NERVOUS SYSTEM AND SECRETORY TISSUES OF DROSOPHILA
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IDENTIFICATION OF A SECOND HOMOLOG OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN THAT IS EXPRESSED IN THE NERVOUS SYSTEM AND SECRETORY TISSUES OF DROSOPHILA

机译：鉴定在果蝇的神经系统和分泌组织中表达的N-乙基丙二酰亚胺敏感融合蛋白的第二个同源物

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摘要

N-Ethylmaleimide-sensitive fusion protein (NSF) is an ATPase known to have an essential role in intracellular membrane transport events. Recently, cDNA clones encoding a Drosophila melanogaster homolog of this protein, named dNSF, were characterized and found to be expressed in the nervous system. We now report the identification of a second homolog of NSF, called dNSF-2 within this species and report evidence that this ubiquitous and widely utilized fusion protein belongs to a multigene family. The predicted amino acid sequence of dNSF-2 is 84.5% identical to dNSF (hereafter named dNSF-1), 59% identical to NSF from Chinese hamster, and 38.5% identical to the yeast homolog SEC18. The highest similarity was found in a region of dNSF-2 containing one of two ATP-binding sites; this region is most similar to members of a superfamily of ATPases. dNSF-2 is localized to a region between bands 87F12 and 88A3 on chromosome 3, and ill situ hybridization techniques revealed expression in the nervous system during embryogenesis and in several imaginal discs and secretory structures in the larvae. Developmental modulation of dNSF-2 expression suggests that quantitative changes in the secretory apparatus are important in histogenesis. [References: 37]
机译：N-乙基马来酰亚胺敏感的融合蛋白（NSF）是一种ATPase，已知在细胞内膜转运事件中具有重要作用。最近，表征该蛋白的果蝇果蝇同源物的cDNA克隆被命名为dNSF，并被发现在神经系统中表达。我们现在报告鉴定该种内NSF的第二个同系物，称为dNSF-2，并报告该无处不在且被广泛利用的融合蛋白属于多基因家族的证据。 dNSF-2的预测氨基酸序列与dNSF（以下称为dNSF-1）相同，为84.5％，与中国仓鼠的NSF相同，为59％，与酵母同源SEC18相同，为38.5％。在包含两个ATP结合位点之一的dNSF-2区域发现了最高的相似性。该区域与ATPase超家族的成员最相似。 dNSF-2位于3号染色体上的87F12和88A3条带之间的区域，而原位杂交技术揭示了胚胎发生过程中在神经系统中以及幼虫中一些假想的椎间盘和分泌结构中的表达。 dNSF-2表达的发育调节表明分泌设备的定量变化在组织发生中很重要。 [参考：37]

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