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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates
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Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

机译：kHz速率下神经元活动的无像差三维多光子成像

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摘要

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.
机译：多光子显微镜是神经科学中的强大工具，有望提供有关单个神经元内以及神经元网络内时空活动的重要数据。当前技术的主要局限性是与在x和y方向上可获得的kHz速率相比，在z方向上的扫描速率相对较慢。在这里，我们描述了一种定制的显微镜系统，该系统基于一种体系结构，该体系结构可在所有三个维度上实现数百微米的kHz扫描速率而不会引入像差。我们进一步演示了如何使用这种高速3D多光子成像系统在亚细胞以及种群水平上以毫秒级的分辨率研究神经元活动。

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  • 作者

    Edward J. Botcherby; Christopher W. Smith; Michael M. Kohl; Delphine Debarre; Martin J. Booth; Rimas Juskaitis; Ole Paulsen; Tony Wilson;

  • 作者单位

    Department of Engineering Science, University of Oxford, Parks Road, Oxford 0X1 3PJ, United Kingdom;

    Department of Engineering Science, University of Oxford, Parks Road, Oxford 0X1 3PJ, United Kingdom;

    Department of Physiology, Anatomy and Genetics, University of Oxford, Parks Road, Oxford 0X1 3PT, United Kingdom,Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EG, United Kingdom;

    Department of Engineering Science, University of Oxford, Parks Road, Oxford 0X1 3PJ, United Kingdom,Laboratory for Optics and Biosciences, Ecole Polytechnique, 91128 Palaiseau, France;

    Department of Engineering Science, University of Oxford, Parks Road, Oxford 0X1 3PJ, United Kingdom;

    Department of Engineering Science, University of Oxford, Parks Road, Oxford 0X1 3PJ, United Kingdom;

    Department of Physiology, Anatomy and Genetics, University of Oxford, Parks Road, Oxford 0X1 3PT, United Kingdom,Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EG, United Kingdom;

    Department of Engineering Science, University of Oxford, Parks Road, Oxford 0X1 3PJ, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    fluorescence microscopy; multiphoton imaging; multiphoton microscopy; three-dimensional microscopy;

    机译：荧光显微镜多光子成像多光子显微镜三维显微镜;

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