Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism

Vladimir Kostal; Petr Simek; Helena Zahradnickova; Jana Cimlova; Tomas Stetina

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Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism

机译：将寒冷敏感的果蝇幼虫（果蝇）转变为耐冻生物

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摘要

Among vertebrates, only a few species of amphibians and reptiles tolerate the formation of ice crystals in their body fluids. Freeze tolerance is much more widespread in invertebrates, especially in overwintering insects. Evolutionary adaptations for freeze tolerance are considered to be highly complex. Here we show that surprisingly simple laboratory manipulations can change the chill susceptible insect to the freeze tolerant one. Larvae of Drosophila melanogaster, a fruit fly of tropical origin with a weak innate capacity to tolerate mild chilling, can survive when approximately 50% of their body water freezes. To achieve this goal, synergy of two fundamental prerequisites is required: (ⅰ) shutdown of larval development by exposing larvae to low temperatures (dormancy) and (ⅱ) incorporating the free amino acid proline in tissues by feeding larvae a proline-augmented diet (cryopreservation).

机译：在脊椎动物中，只有极少数的两栖动物和爬行动物能够忍受其体液中冰晶的形成。在无脊椎动物中，尤其是在越冬昆虫中，抗冻性更为普遍。冷冻耐受性的进化适应被认为是高度复杂的。在这里，我们表明出乎意料的简单实验室操作可以将对寒冷敏感的昆虫改变为耐冷冻的昆虫。果蝇果蝇（Drosophila melanogaster）的幼虫是一种热带起源的果蝇，其先天能力较弱，耐温和的寒冷，当其体内约有50％的水冻结时可以存活。为了实现这一目标，需要两个基本先决条件的协同作用：（ⅰ）通过使幼虫处于低温状态（休眠状态）来关闭幼虫的发育；（ⅱ）通过给幼虫喂食增加了脯氨酸的饮食来将游离氨基酸脯氨酸掺入组织中（冷冻保存）。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2012年第9期|p.3270-3274|共5页
作者
Vladimir Kostal; Petr Simek; Helena Zahradnickova; Jana Cimlova; Tomas Stetina;
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作者单位

Institute of Entomology, Biology Centre ASCR (Academy of Sciences of the Czech Republic), Branisovska 31, 370 05 Ceske Budejovice, Czech Republic,Faculty of Science, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic;

Institute of Entomology, Biology Centre ASCR (Academy of Sciences of the Czech Republic), Branisovska 31, 370 05 Ceske Budejovice, Czech Republic;

Institute of Entomology, Biology Centre ASCR (Academy of Sciences of the Czech Republic), Branisovska 31, 370 05 Ceske Budejovice, Czech Republic;

Institute of Entomology, Biology Centre ASCR (Academy of Sciences of the Czech Republic), Branisovska 31, 370 05 Ceske Budejovice, Czech Republic;

Faculty of Science, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
insect cold tolerance; long-term storage; metabolomics; cryoprotection; quiescence;

机译：昆虫耐寒性长期存放;代谢组学防冻静止;

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Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism

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