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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genome-wide analysis of histone H3.1 and H3.3 variants in Arabidopsis thaliana
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Genome-wide analysis of histone H3.1 and H3.3 variants in Arabidopsis thaliana

机译：拟南芥中组蛋白H3.1和H3.3变异的全基因组分析

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摘要

Nucleosomes package eukaryotic DNA and are composed of four different histone proteins, designated H3, H4, H2A, and H2B. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. We profiled H3.1 and H3.3 variants in the genome of the plant Arabidopsis thaliana and found that the localization of these variants shows broad similarity in plants and animals, along with some unique features. H3.1 was enriched in silent areas of the genome, including regions containing the repressive chromatin modifications H3 lysine 27 methylation, H3 lysine 9 methylation, and DNA methylation. In contrast, H3.3 was enriched in actively transcribed genes, especially peaking at the 3' end of genes, and correlated with histone modifications associated with gene activation, such as histone H3 lysine 4 methylation and H2B ubiquitylation, as well as RNA Pol II occupancy. Surprisingly, both H3.1 and H3.3 were enriched on defined origins of replication, as was overall nucleosome density, suggesting a novel characteristic of plant origins. Our results are broadly consistent with the hypothesis that H3.1 acts as the canonical histone that is incorporated during ONA replication, whereas H3.3 acts as the replacement histone that can be incorporated outside of S-phase during chromatin-disrupting processes like transcription.
机译：核小体包装真核生物DNA，由四种不同的组蛋白组成，称为H3，H4，H2A和H2B。组蛋白H3具有两个主要变体H3.1和H3.3，它们在动物中显示出不同的基因组定位模式。我们在植物拟南芥基因组中分析了H3.1和H3.3变体，发现这些变体的定位在动植物中显示出广泛的相似性，并具有一些独特的功能。 H3.1富集在基因组的沉默区域，包括含有抑制性染色质修饰的区域H3赖氨酸27甲基化，H3赖氨酸9甲基化和DNA甲基化。相反，H3.3富含活跃转录的基因，尤其是在基因的3'末端达到峰值，并与与基因激活相关的组蛋白修饰相关，例如组蛋白H3赖氨酸4甲基化和H2B泛素化，以及RNA Pol II占用。出乎意料的是，H3.1和H3.3都在确定的复制起点上富集，总的核小体密度也如此，这表明植物起点具有新特征。我们的结果与以下假设基本一致：H3.1充当ONA复制过程中掺入的规范组蛋白，而H3.3充当可以在染色质破坏过程（如转录）中掺入S相之外的替代组蛋白。

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    Hume Stroud; Sofia Otero; Benedicte Desvoyes; Elena Ramirez-Parra; Steven E. Jacobsen; Crisanto Gutierrez;

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    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095;

    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientif icas, Universidad Autonoma de Madrid, Nicolas Cabrera 1, 28049 Madrid, Spain;

    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientif icas, Universidad Autonoma de Madrid, Nicolas Cabrera 1, 28049 Madrid, Spain;

    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientif icas, Universidad Autonoma de Madrid, Nicolas Cabrera 1, 28049 Madrid, Spain,Centro de Biotecnologia y Genomica de Plantas, Campus de Montegancedo, 28223 Madrid, Spain;

    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095,Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095,Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, University of California, Los Angeles, CA 90095;

    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientif icas, Universidad Autonoma de Madrid, Nicolas Cabrera 1, 28049 Madrid, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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